Analysis of erythrocyte invasion mechanisms of plasmodium falciparum clinical isolates across 3 malaria-endemic areas in Ghana

Henrietta E. Mensah-Brown, Nicholas Amoako, James Abugri, Lindsay B. Stewart, Godfred Agongo, Emmanuel K. Dickson, Michael F. Ofori, José A. Stoute, David J. Conway, Gordon A. Awandare

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background.Plasmodium falciparum invades human erythrocytes by using an array of ligands that interact with several receptors, including sialic acid (SA), complement receptor 1 (CR1), and basigin. We hypothesized that in malaria-endemic areas, parasites vary invasion pathways under immune pressure. Therefore, invasion mechanisms of clinical isolates collected from 3 zones of Ghana with different levels of endemicity (from lowest to highest, Accra, Navrongo, and Kintampo) were compared using standardized methods. Methods.Blood samples were collected from children aged 2-14 years in whom malaria was diagnosed, and erythrocyte invasion phenotypes were determined using the enzymes neuraminidase, chymotrypsin, and trypsin, which differentially cleave receptors from the erythrocyte surface. In addition, antibodies against CR1 and basigin were used to determine the contributions of these receptors to invasion. Gene expression levels of P. falciparum invasion ligands were also examined. Results.The parasites generally expressed SA-independent invasion phenotypes across the malaria-endemic areas, with parasites from Kintampo showing the highest invasion rates in neuraminidase-treated erythrocytes. CR1 was a major mediator of SA-independent invasion, while basigin was essential for both SA-dependent and SA-independent invasion mechanisms. Furthermore, expression of the basigin ligand PfRh5 was the best predictor of donor parasitemia. Conclusions.Erythrocyte invasion phenotypes expressed by P. falciparum are influenced by endemicity levels, and the PfRh5-basigin pathway is a potential vaccine target.

Original languageEnglish (US)
Pages (from-to)1288-1297
Number of pages10
JournalJournal of Infectious Diseases
Volume212
Issue number8
DOIs
StatePublished - Oct 15 2015

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CD147 Antigens
Ghana
Plasmodium falciparum
Malaria
N-Acetylneuraminic Acid
Complement C1
Complement Receptors
Erythrocytes
Parasites
Neuraminidase
Ligands
Phenotype
Parasitemia
Vaccines
Tissue Donors
Gene Expression
Pressure
Antibodies
Enzymes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Mensah-Brown, H. E., Amoako, N., Abugri, J., Stewart, L. B., Agongo, G., Dickson, E. K., ... Awandare, G. A. (2015). Analysis of erythrocyte invasion mechanisms of plasmodium falciparum clinical isolates across 3 malaria-endemic areas in Ghana. Journal of Infectious Diseases, 212(8), 1288-1297. https://doi.org/10.1093/infdis/jiv207
Mensah-Brown, Henrietta E. ; Amoako, Nicholas ; Abugri, James ; Stewart, Lindsay B. ; Agongo, Godfred ; Dickson, Emmanuel K. ; Ofori, Michael F. ; Stoute, José A. ; Conway, David J. ; Awandare, Gordon A. / Analysis of erythrocyte invasion mechanisms of plasmodium falciparum clinical isolates across 3 malaria-endemic areas in Ghana. In: Journal of Infectious Diseases. 2015 ; Vol. 212, No. 8. pp. 1288-1297.
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Mensah-Brown, HE, Amoako, N, Abugri, J, Stewart, LB, Agongo, G, Dickson, EK, Ofori, MF, Stoute, JA, Conway, DJ & Awandare, GA 2015, 'Analysis of erythrocyte invasion mechanisms of plasmodium falciparum clinical isolates across 3 malaria-endemic areas in Ghana', Journal of Infectious Diseases, vol. 212, no. 8, pp. 1288-1297. https://doi.org/10.1093/infdis/jiv207

Analysis of erythrocyte invasion mechanisms of plasmodium falciparum clinical isolates across 3 malaria-endemic areas in Ghana. / Mensah-Brown, Henrietta E.; Amoako, Nicholas; Abugri, James; Stewart, Lindsay B.; Agongo, Godfred; Dickson, Emmanuel K.; Ofori, Michael F.; Stoute, José A.; Conway, David J.; Awandare, Gordon A.

In: Journal of Infectious Diseases, Vol. 212, No. 8, 15.10.2015, p. 1288-1297.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Analysis of erythrocyte invasion mechanisms of plasmodium falciparum clinical isolates across 3 malaria-endemic areas in Ghana

AU - Mensah-Brown, Henrietta E.

AU - Amoako, Nicholas

AU - Abugri, James

AU - Stewart, Lindsay B.

AU - Agongo, Godfred

AU - Dickson, Emmanuel K.

AU - Ofori, Michael F.

AU - Stoute, José A.

AU - Conway, David J.

AU - Awandare, Gordon A.

PY - 2015/10/15

Y1 - 2015/10/15

N2 - Background.Plasmodium falciparum invades human erythrocytes by using an array of ligands that interact with several receptors, including sialic acid (SA), complement receptor 1 (CR1), and basigin. We hypothesized that in malaria-endemic areas, parasites vary invasion pathways under immune pressure. Therefore, invasion mechanisms of clinical isolates collected from 3 zones of Ghana with different levels of endemicity (from lowest to highest, Accra, Navrongo, and Kintampo) were compared using standardized methods. Methods.Blood samples were collected from children aged 2-14 years in whom malaria was diagnosed, and erythrocyte invasion phenotypes were determined using the enzymes neuraminidase, chymotrypsin, and trypsin, which differentially cleave receptors from the erythrocyte surface. In addition, antibodies against CR1 and basigin were used to determine the contributions of these receptors to invasion. Gene expression levels of P. falciparum invasion ligands were also examined. Results.The parasites generally expressed SA-independent invasion phenotypes across the malaria-endemic areas, with parasites from Kintampo showing the highest invasion rates in neuraminidase-treated erythrocytes. CR1 was a major mediator of SA-independent invasion, while basigin was essential for both SA-dependent and SA-independent invasion mechanisms. Furthermore, expression of the basigin ligand PfRh5 was the best predictor of donor parasitemia. Conclusions.Erythrocyte invasion phenotypes expressed by P. falciparum are influenced by endemicity levels, and the PfRh5-basigin pathway is a potential vaccine target.

AB - Background.Plasmodium falciparum invades human erythrocytes by using an array of ligands that interact with several receptors, including sialic acid (SA), complement receptor 1 (CR1), and basigin. We hypothesized that in malaria-endemic areas, parasites vary invasion pathways under immune pressure. Therefore, invasion mechanisms of clinical isolates collected from 3 zones of Ghana with different levels of endemicity (from lowest to highest, Accra, Navrongo, and Kintampo) were compared using standardized methods. Methods.Blood samples were collected from children aged 2-14 years in whom malaria was diagnosed, and erythrocyte invasion phenotypes were determined using the enzymes neuraminidase, chymotrypsin, and trypsin, which differentially cleave receptors from the erythrocyte surface. In addition, antibodies against CR1 and basigin were used to determine the contributions of these receptors to invasion. Gene expression levels of P. falciparum invasion ligands were also examined. Results.The parasites generally expressed SA-independent invasion phenotypes across the malaria-endemic areas, with parasites from Kintampo showing the highest invasion rates in neuraminidase-treated erythrocytes. CR1 was a major mediator of SA-independent invasion, while basigin was essential for both SA-dependent and SA-independent invasion mechanisms. Furthermore, expression of the basigin ligand PfRh5 was the best predictor of donor parasitemia. Conclusions.Erythrocyte invasion phenotypes expressed by P. falciparum are influenced by endemicity levels, and the PfRh5-basigin pathway is a potential vaccine target.

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