Analysis of genetic bottlenecks during horizontal transmission of Cucumber Mosaic Virus

Akhtar Ali, Hongye Li, William L. Schneider, Diana J. Sherman, Stewart Gray, Dawn Smith, Marilyn J. Roossinck

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

Genetic bottlenecks may occur in virus populations when only a few individuals are transferred horizontally from one host to another, or when a viral population moves systemically from the infection site. Genetic bottlenecks during the systemic movement of an RNA plant virus population were reported previously (H. Li and M. J. Roossinck, J. Virol. 78:10582-10587, 2004). In this study we mechanically inoculated an artificial population consisting of 12 restriction enzyme marker mutants of Cucumber mosaic virus (CMV) onto young leaves of squash plants and used two aphid species, Aphis gossypii and Myzus persicae, to transmit the virus populations from infected source plants to healthy squash plants. Horizontal transmission by aphids constituted a significant bottleneck, as the population in the aphid-inoculated plants contained far fewer mutants than the original inoculum source. Additional experiments demonstrated that genetic variation in the artificial population of CMV is not reduced during the acquisition of the virus but is significantly reduced during the inoculation period.

Original languageEnglish (US)
Pages (from-to)8345-8350
Number of pages6
JournalJournal of virology
Volume80
Issue number17
DOIs
StatePublished - Sep 1 2006

Fingerprint

Cucumovirus
Cucumber mosaic virus
genetic techniques and protocols
Aphids
Population
Aphidoidea
Cucurbita
squashes
Viruses
viruses
Plant Viruses
mutants
Aphis gossypii
RNA Viruses
plant viruses
Myzus persicae
inoculum
RNA
genetic variation
Enzymes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Ali, Akhtar ; Li, Hongye ; Schneider, William L. ; Sherman, Diana J. ; Gray, Stewart ; Smith, Dawn ; Roossinck, Marilyn J. / Analysis of genetic bottlenecks during horizontal transmission of Cucumber Mosaic Virus. In: Journal of virology. 2006 ; Vol. 80, No. 17. pp. 8345-8350.
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Analysis of genetic bottlenecks during horizontal transmission of Cucumber Mosaic Virus. / Ali, Akhtar; Li, Hongye; Schneider, William L.; Sherman, Diana J.; Gray, Stewart; Smith, Dawn; Roossinck, Marilyn J.

In: Journal of virology, Vol. 80, No. 17, 01.09.2006, p. 8345-8350.

Research output: Contribution to journalArticle

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Ali A, Li H, Schneider WL, Sherman DJ, Gray S, Smith D et al. Analysis of genetic bottlenecks during horizontal transmission of Cucumber Mosaic Virus. Journal of virology. 2006 Sep 1;80(17):8345-8350. https://doi.org/10.1128/JVI.00568-06