Analysis of mammalian cell cytoplasm with electrophoresis in nanometer inner diameter capillaries

Lori A. Woods, Paula R. Powell, Tracy L. Paxon, Andrew G. Ewing

Research output: Contribution to journalArticlepeer-review

Abstract

Capillary electrophoresis in 770 nanometer inner diameter capillaries coupled to electrochemical detection with an etched electrode matching an etched capillary (etched electrochemical detection) has been used with ultrasmall sampling to inject subcellular samples from intact single mammalian cells. Separations of cytoplasmic samples taken from rat pheochromocytoma cells have been achieved. As little as 8% of the total volume of a single cell has been sampled and analyzed. Dopamine has been identified and quantified in these PC12 cells using this technique. The average cytoplasmic level of dopamine in rat pheochromocytoma cells has been determined to be 240±60 μM. The use of electrophoresis in 770 nanometer inner diameter capillaries with electrochemical detection to monitor cytoplasmic neurotransmitters at the single cell level can provide information about complex cellular functions such as neurotransmitter storage and synthesis.

Original languageEnglish (US)
Pages (from-to)1192-1197
Number of pages6
JournalElectroanalysis
Volume17
Issue number13
DOIs
StatePublished - Jul 2005

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Electrochemistry

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