Analysis of single cells with capillary electrophoresis electrospray ionization Fourier transform ion cycloton resonance mass spectrometry

Steven A. Hofstadler, Joanne C. Severs, Richard D. Smith, Franklin D. Swanek, Andrew G. Ewing

Research output: Contribution to journalArticlepeer-review

Abstract

Recent results with on-line capillary electrophoresis (CE) electrospray ionization (ESI) Fourier transform ion cyclotron resonance (FTICR) mass spectrometry suggest that CE/ESI-FTICR can provide a powerful technique for micro-sample analyses owing to the inherent sensitivity of the technique and the enhanced information content derived from high-performance mass measurements. Using micro-sampling methods and ion accumulation techniques based on quadrupolar excitation, we demonstrate that adequate sensitivity exists to characterize the hemoglobin from a single human erythrocyte (≈450 amol). In these studies mass spectra with average mass resolution in excess of 45 000 (FWHM) were obtained for both the α- and β chain of hemoglobin following in-column lysing of a single erythrocyte.

Original languageEnglish (US)
Pages (from-to)919-922
Number of pages4
JournalRapid Communications in Mass Spectrometry
Volume10
Issue number8
DOIs
StatePublished - 1996

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Spectroscopy
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Analysis of single cells with capillary electrophoresis electrospray ionization Fourier transform ion cycloton resonance mass spectrometry'. Together they form a unique fingerprint.

Cite this