Recent results with on-line capillary electrophoresis (CE) electrospray ionization (ESI) Fourier transform ion cyclotron resonance (FTICR) mass spectrometry suggest that CE/ESI-FTICR can provide a powerful technique for micro-sample analyses owing to the inherent sensitivity of the technique and the enhanced information content derived from high-performance mass measurements. Using micro-sampling methods and ion accumulation techniques based on quadrupolar excitation, we demonstrate that adequate sensitivity exists to characterize the hemoglobin from a single human erythrocyte (≈450 amol). In these studies mass spectra with average mass resolution in excess of 45 000 (FWHM) were obtained for both the α- and β chain of hemoglobin following in-column lysing of a single erythrocyte.
|Original language||English (US)|
|Number of pages||4|
|Journal||Rapid Communications in Mass Spectrometry|
|State||Published - 1996|
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Organic Chemistry