Analysis of the complete mitochondrial genome sequences of the soybean rust pathogens Phakopsora pachyrhizi and P. meibomiae

Christine L. Stone, Martha Lucia Posada Buitrago, Jeffrey L. Boore, Reid D. Frederick

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The mitochondrial (mt) genomes of two soybean rust pathogens, Phakopsora pachyrhizi and P. meibomiae, have been sequenced. The mt genome of P. pachyrhizi is a circular 31 825-bp molecule with a mean GC content of 34.6%, while P. meibomiae possesses a 32 520-bp circular molecule with a mean GC content of 34.9%. Both mt genomes contain the genes encoding ATP synthase subunits 6, 8 and 9 (atp6, atp8 and atp9), cytochrome oxidase subunits I, II and III (cox1, cox2 and cox3), apocytochrome b (cob), reduced nicotinamide adenine dinucleotide ubiquinone oxidoreductase subunits (nad1, nad2, nad3, nad4, nad4L, nad5 and nad6), the large and small mt ribosomal RNA genes, one ORF coding for a ribosomal protein (rps3), and a set of 24 tRNA genes that recognize codons for all amino acids. The order of the protein-coding genes and tRNA is identical in the two Phakopsora species, and all genes are transcribed from the same DNA strand clockwise. Introns were identified in the cox1, cob and rnl genes of both species, with three of the introns having ORFs with motifs similar to the LAGLIDADG endonu-cleases of other fungi. Phylogenetic analysis of the 14 shared protein-coding genes agrees with commonly accepted fungal taxonomy.

Original languageEnglish (US)
Pages (from-to)887-897
Number of pages11
JournalMycologia
Volume102
Issue number4
DOIs
StatePublished - Jul 1 2010

Fingerprint

Phakopsora pachyrhizi
soybean rust
Mitochondrial Genome
rust disease
Soybeans
soybean
genome
pathogen
gene
pathogens
Base Composition
Transfer RNA
Introns
Open Reading Frames
Genes
genes
Cytochromes b
Ubiquinone
Mitochondrial Genes
Ribosomal Proteins

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Stone, Christine L. ; Buitrago, Martha Lucia Posada ; Boore, Jeffrey L. ; Frederick, Reid D. / Analysis of the complete mitochondrial genome sequences of the soybean rust pathogens Phakopsora pachyrhizi and P. meibomiae. In: Mycologia. 2010 ; Vol. 102, No. 4. pp. 887-897.
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Analysis of the complete mitochondrial genome sequences of the soybean rust pathogens Phakopsora pachyrhizi and P. meibomiae. / Stone, Christine L.; Buitrago, Martha Lucia Posada; Boore, Jeffrey L.; Frederick, Reid D.

In: Mycologia, Vol. 102, No. 4, 01.07.2010, p. 887-897.

Research output: Contribution to journalArticle

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