Analysis of the human intestinal epithelial cell transcriptional response to Lactobacillus acidophilus, Lactobacillus salivarius, Bifidobacterium lactis and Escherichia coli

H. Putaala, Rodolphe Barrangou, G. J. Leyer, A. C. Ouwehand, E. Bech Hansen, D. A. Romero, N. Rautonen

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The complex microbial population residing in the human gastrointestinal tract consists of commensal, potential pathogenic and beneficial species, which are probably perceived differently by the host and consequently could be expected to trigger specific transcriptional responses. Here, we provide a comparative analysis of the global in vitro transcriptional response of human intestinal epithelial cells to Lactobacillus acidophilus NCFM™, Lactobacillus salivarius Ls-33, Bifidobacterium animalis subsp. lactis 420, and enterohaemorrhagic Escherichia coli O157:H7 (EHEC). Interestingly, L. salivarius Ls-33 DCE-induced changes were overall more similar to those of B. lactis 420 than to L. acidophilus NCFM™, which is consistent with previously observed in vivo immunomodulation properties. In the gene ontology and pathway analyses both specific and unspecific changes were observed. Common to all was the regulation of apoptosis and adipogenesis, and lipid-metabolism related regulation by the probiotics. Specific changes such as regulation of cell-cell adhesion by B. lactis 420, superoxide metabolism by L. salivarius Ls-33, and regulation of MAPK pathway by L. acidophilus NCFM™ were noted. Furthermore, fundamental differences were observed between the pathogenic and probiotic treatments in the Toll-like receptor pathway, especially for adapter molecules with a lowered level of transcriptional activation of MyD88, TRIF, IRAK1 and TRAF6 by probiotics compared to EHEC. The results in this study provide insights into the relationship between probiotics and human intestinal epithelial cells, notably with regard to strain-specific responses, and highlight the differences between transcriptional responses to pathogenic and probiotic bacteria.

Original languageEnglish (US)
Pages (from-to)283-295
Number of pages13
JournalBeneficial Microbes
Volume1
Issue number3
DOIs
StatePublished - Jan 1 2010

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Lactobacillus acidophilus
Probiotics
Epithelial Cells
Escherichia coli
Enterohemorrhagic Escherichia coli
Escherichia coli O157
TNF Receptor-Associated Factor 6
Adipogenesis
Gene Ontology
Immunomodulation
Toll-Like Receptors
Lipid Metabolism
Cell Adhesion
Superoxides
Transcriptional Activation
Gastrointestinal Tract
Bifidobacterium animalis
Lactobacillus salivarius
Apoptosis
Bacteria

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

Putaala, H. ; Barrangou, Rodolphe ; Leyer, G. J. ; Ouwehand, A. C. ; Bech Hansen, E. ; Romero, D. A. ; Rautonen, N. / Analysis of the human intestinal epithelial cell transcriptional response to Lactobacillus acidophilus, Lactobacillus salivarius, Bifidobacterium lactis and Escherichia coli. In: Beneficial Microbes. 2010 ; Vol. 1, No. 3. pp. 283-295.
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Analysis of the human intestinal epithelial cell transcriptional response to Lactobacillus acidophilus, Lactobacillus salivarius, Bifidobacterium lactis and Escherichia coli. / Putaala, H.; Barrangou, Rodolphe; Leyer, G. J.; Ouwehand, A. C.; Bech Hansen, E.; Romero, D. A.; Rautonen, N.

In: Beneficial Microbes, Vol. 1, No. 3, 01.01.2010, p. 283-295.

Research output: Contribution to journalArticle

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