Analysis of the phospholipid of the nuclear envelope and endoplasmic reticulum of liver cells by high pressure liquid chromatography

Jacqueline L. James, Gary A. Clawson, Christine H. Chan, Edward A. Smuckler

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

A method is described for the separation and analysis of phospholipids from rat-liver nuclear envelope and endoplasmic reticulum. The procedure employs a liquid environment, to which antioxidants can be added, and results in separation of NL, PE, PI, PS, and PC in 99% purity in 12 min; analytical columns and a radial compression system may be employed. The procedure results in phospholipids with a large proportion of highly unsaturated fatty acids; some differences in fatty acid distributions were found when nuclear envelope phospholipid fractions were compared with the corresponding fractions from endoplasmic reticulum.

Original languageEnglish (US)
Pages (from-to)541-545
Number of pages5
JournalLipids
Volume16
Issue number7
DOIs
StatePublished - Jul 1981

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

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