Analysis of the phospholipid of the nuclear envelope and endoplasmic reticulum of liver cells by high pressure liquid chromatography

Jacqueline L. James; Gary A. Clawson; Christine H. Chan; Edward A. Smuckler

doi:10.1007/BF02535053

Analysis of the phospholipid of the nuclear envelope and endoplasmic reticulum of liver cells by high pressure liquid chromatography

Jacqueline L. James, Gary A. Clawson, Christine H. Chan, Edward A. Smuckler

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27 Scopus citations

Abstract

A method is described for the separation and analysis of phospholipids from rat-liver nuclear envelope and endoplasmic reticulum. The procedure employs a liquid environment, to which antioxidants can be added, and results in separation of NL, PE, PI, PS, and PC in 99% purity in 12 min; analytical columns and a radial compression system may be employed. The procedure results in phospholipids with a large proportion of highly unsaturated fatty acids; some differences in fatty acid distributions were found when nuclear envelope phospholipid fractions were compared with the corresponding fractions from endoplasmic reticulum.

Original language	English (US)
Pages (from-to)	541-545
Number of pages	5
Journal	Lipids
Volume	16
Issue number	7
DOIs	https://doi.org/10.1007/BF02535053
State	Published - Jul 1981

All Science Journal Classification (ASJC) codes

Biochemistry
Organic Chemistry
Cell Biology

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10.1007/BF02535053

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abstract = "A method is described for the separation and analysis of phospholipids from rat-liver nuclear envelope and endoplasmic reticulum. The procedure employs a liquid environment, to which antioxidants can be added, and results in separation of NL, PE, PI, PS, and PC in 99% purity in 12 min; analytical columns and a radial compression system may be employed. The procedure results in phospholipids with a large proportion of highly unsaturated fatty acids; some differences in fatty acid distributions were found when nuclear envelope phospholipid fractions were compared with the corresponding fractions from endoplasmic reticulum.",

author = "James, {Jacqueline L.} and Clawson, {Gary A.} and Chan, {Christine H.} and Smuckler, {Edward A.}",

year = "1981",

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AU - James, Jacqueline L.

AU - Clawson, Gary A.

AU - Chan, Christine H.

AU - Smuckler, Edward A.

PY - 1981/7

Y1 - 1981/7

N2 - A method is described for the separation and analysis of phospholipids from rat-liver nuclear envelope and endoplasmic reticulum. The procedure employs a liquid environment, to which antioxidants can be added, and results in separation of NL, PE, PI, PS, and PC in 99% purity in 12 min; analytical columns and a radial compression system may be employed. The procedure results in phospholipids with a large proportion of highly unsaturated fatty acids; some differences in fatty acid distributions were found when nuclear envelope phospholipid fractions were compared with the corresponding fractions from endoplasmic reticulum.

AB - A method is described for the separation and analysis of phospholipids from rat-liver nuclear envelope and endoplasmic reticulum. The procedure employs a liquid environment, to which antioxidants can be added, and results in separation of NL, PE, PI, PS, and PC in 99% purity in 12 min; analytical columns and a radial compression system may be employed. The procedure results in phospholipids with a large proportion of highly unsaturated fatty acids; some differences in fatty acid distributions were found when nuclear envelope phospholipid fractions were compared with the corresponding fractions from endoplasmic reticulum.

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Analysis of the phospholipid of the nuclear envelope and endoplasmic reticulum of liver cells by high pressure liquid chromatography

Abstract

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