Analysis of the phospholipid of the nuclear envelope and endoplasmic reticulum of liver cells by high pressure liquid chromatography

Jacqueline L. James, Gary Clawson, Christine H. Chan, Edward A. Smuckler

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

A method is described for the separation and analysis of phospholipids from rat-liver nuclear envelope and endoplasmic reticulum. The procedure employs a liquid environment, to which antioxidants can be added, and results in separation of NL, PE, PI, PS, and PC in 99% purity in 12 min; analytical columns and a radial compression system may be employed. The procedure results in phospholipids with a large proportion of highly unsaturated fatty acids; some differences in fatty acid distributions were found when nuclear envelope phospholipid fractions were compared with the corresponding fractions from endoplasmic reticulum.

Original languageEnglish (US)
Pages (from-to)541-545
Number of pages5
JournalLipids
Volume16
Issue number7
DOIs
StatePublished - Jul 1 1981

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High pressure liquid chromatography
Nuclear Envelope
nuclear membrane
Endoplasmic Reticulum
Liver
endoplasmic reticulum
hepatocytes
Phospholipids
phospholipids
high performance liquid chromatography
High Pressure Liquid Chromatography
highly unsaturated fatty acids
Unsaturated Fatty Acids
purity
Rats
Fatty Acids
Antioxidants
fatty acids
antioxidants
liver

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

Cite this

James, Jacqueline L. ; Clawson, Gary ; Chan, Christine H. ; Smuckler, Edward A. / Analysis of the phospholipid of the nuclear envelope and endoplasmic reticulum of liver cells by high pressure liquid chromatography. In: Lipids. 1981 ; Vol. 16, No. 7. pp. 541-545.
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Analysis of the phospholipid of the nuclear envelope and endoplasmic reticulum of liver cells by high pressure liquid chromatography. / James, Jacqueline L.; Clawson, Gary; Chan, Christine H.; Smuckler, Edward A.

In: Lipids, Vol. 16, No. 7, 01.07.1981, p. 541-545.

Research output: Contribution to journalArticle

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