Analysis of the Pvull Restriction Fragment-length Polymorphism and Exon Structure of the Estrogen Receptor Gene in Breast Cancer and Peripheral Blood

Lauren Yaich, William D. Dupont, Douglas R. Cavener, Fritz F. Pari

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223 Scopus citations

Abstract

The presence of estrogen receptor (ER) is a well-known predictor of clinical outcome in human breast cancer. We examined the ER gene in 26 primary breast cancers (14 ER-positive, 12 ER-negative) to determine if alterations of the gene are associated with the ER-negative status. In tumor biopsies and peripheral blood DNA obtained from the same patients we analyzed the ER exon structure using polymerase chain reaction amplification, restriction endonuclease digestion, and agarose gel electrophoresis. All blood and tumor samples, regardless of ER status, showed a complete set of eight exons of normal sizes, ruling out deletions or rearrangements of the ER gene in excess of ±20 nucleotides. Previous reports indicate that the two-allele ER Pvull polymorphism could be associated with ER expression in breast cancer (Hill et al., Cancer Res., 49:145-148,1989) as well as with patient age at time of tumor diagnosis (Pari et al., Breast Cancer Res. Treat., 14: 57-64, 1989). We localized the Pvull polymorphism in intron 1, 0.4 kilobase upstream of exon 2. Sequence analysis showed the polymorphism to result from a point mutation (T-*C) at the fifth position of the restriction site (CATCiTG). We determined the Pvull restriction fragment-length polymorphism genotype in 257 primary breast cancers and 140 peripheral blood DNA samples obtained from women without breast cancer. The results indicate that the Pvull polymorphism is not associated with ER content or patient age at tumor diagnosis.

Original languageEnglish (US)
Pages (from-to)77-83
Number of pages7
JournalCancer Research
Volume52
Issue number1
StatePublished - Jan 1992

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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