Analytical considerations for quantitative determination of serotonin and its metabolically related products in biological matrices

Richard Mailman, C. D. Kilts

Research output: Contribution to journalArticle

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Abstract

We discuss the use of 'high-performance' liquid chromatography with electrochemical detection for the quantification of serotonin (5-hydroxytryptamine) and metabolically related products (i.e., 5-hydroxyindoleacetic acid and 5-hydroxytryptophan) in biological matrices. Two methods are described: one for routine quantification of nanogram amounts or more; the other a two-column purification-separation ('trace enrichment') technique for determining subnanogram amounts of these compounds. Various factors affecting the liquid-chromatographic separation and quantification of 5-hydroxyindoles are detailed, including selection of appropriate internal standard, sample purification, and effects of changes in mobile phase composition, e.g., ion-pair reagent, organic modifier content, and pH. We describe in detail the trace-enrichment method for determinations in the range of 25 pg to 25 ng, and discuss the general factors to consider when developing or modifying an assay for specific applications.

Original languageEnglish (US)
Pages (from-to)1849-1854
Number of pages6
JournalClinical chemistry
Volume31
Issue number11
DOIs
StatePublished - Jan 1 1985

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All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Biochemistry, medical

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