Analytical Py-GC/MS of Genetically Modified Poplar for the Increased Production of Bio-aromatics

Gorugantu SriBala, Hilal Ezgi Toraman, Steffen Symoens, Annabelle Déjardin, Gilles Pilate, Wout Boerjan, Frederik Ronsse, Kevin M. Van Geem, Guy B. Marin

Research output: Contribution to journalArticle

Abstract

Genetic engineering is a powerful tool to steer bio-oil composition towards the production of speciality chemicals such as guaiacols, syringols, phenols, and vanillin through well-defined biomass feedstocks. Our previous work demonstrated the effects of lignin biosynthesis gene modification on the pyrolysis vapour compositions obtained from wood derived from greenhouse-grown poplars. In this study, field-grown poplars downregulated in the genes encoding CINNAMYL ALCOHOL DEHYDROGENASE (CAD), CAFFEIC ACID O-METHYLTRANSFERASE (COMT) and CAFFEOYL-CoA O-METHYLTRANSFERASE (CCoAOMT), and their corresponding wild type were pyrolysed in a Py-GC/MS. This work aims at capturing the effects of downregulation of the three enzymes on bio-oil composition using principal component analysis (PCA). 3,5-methoxytoluene, vanillin, coniferyl alcohol, 4-vinyl guaiacol, syringol, syringaldehyde, and guaiacol are the determining factors in the PCA analysis that are the substantially affected by COMT, CAD and CCoAOMT enzyme downregulation. COMT and CAD downregulated transgenic lines proved to be statistically different from the wild type because of a substantial difference in S and G lignin units. The sCAD line lead to a significant drop (nearly 51%) in S-lignin derived compounds, while CCoAOMT downregulation affected the least (7–11%). Further, removal of extractives via pretreatment enhanced the statistical differences among the CAD transgenic lines and its wild type. On the other hand, COMT downregulation caused 2-fold reduction in S-derived compounds compared to G-derived compounds. This study manifests the applicability of PCA analysis in tracking the biological changes in biomass (poplar in this case) and their effects on pyrolysis-oil compositions.

Original languageEnglish (US)
Pages (from-to)599-610
Number of pages12
JournalComputational and Structural Biotechnology Journal
Volume17
DOIs
StatePublished - 2019

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caffeate O-methyltransferase
Guaiacol
Lignin
Alcohols
Coenzyme A
Down-Regulation
Principal component analysis
Oils
Principal Component Analysis
Chemical analysis
Acids
Biomass
Pyrolysis
Genetic engineering
Enzymes
Gene encoding
Phenols
Biosynthesis
Greenhouses
Feedstocks

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Computer Science Applications

Cite this

SriBala, Gorugantu ; Toraman, Hilal Ezgi ; Symoens, Steffen ; Déjardin, Annabelle ; Pilate, Gilles ; Boerjan, Wout ; Ronsse, Frederik ; Van Geem, Kevin M. ; Marin, Guy B. / Analytical Py-GC/MS of Genetically Modified Poplar for the Increased Production of Bio-aromatics. In: Computational and Structural Biotechnology Journal. 2019 ; Vol. 17. pp. 599-610.
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Analytical Py-GC/MS of Genetically Modified Poplar for the Increased Production of Bio-aromatics. / SriBala, Gorugantu; Toraman, Hilal Ezgi; Symoens, Steffen; Déjardin, Annabelle; Pilate, Gilles; Boerjan, Wout; Ronsse, Frederik; Van Geem, Kevin M.; Marin, Guy B.

In: Computational and Structural Biotechnology Journal, Vol. 17, 2019, p. 599-610.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Analytical Py-GC/MS of Genetically Modified Poplar for the Increased Production of Bio-aromatics

AU - SriBala, Gorugantu

AU - Toraman, Hilal Ezgi

AU - Symoens, Steffen

AU - Déjardin, Annabelle

AU - Pilate, Gilles

AU - Boerjan, Wout

AU - Ronsse, Frederik

AU - Van Geem, Kevin M.

AU - Marin, Guy B.

PY - 2019

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AB - Genetic engineering is a powerful tool to steer bio-oil composition towards the production of speciality chemicals such as guaiacols, syringols, phenols, and vanillin through well-defined biomass feedstocks. Our previous work demonstrated the effects of lignin biosynthesis gene modification on the pyrolysis vapour compositions obtained from wood derived from greenhouse-grown poplars. In this study, field-grown poplars downregulated in the genes encoding CINNAMYL ALCOHOL DEHYDROGENASE (CAD), CAFFEIC ACID O-METHYLTRANSFERASE (COMT) and CAFFEOYL-CoA O-METHYLTRANSFERASE (CCoAOMT), and their corresponding wild type were pyrolysed in a Py-GC/MS. This work aims at capturing the effects of downregulation of the three enzymes on bio-oil composition using principal component analysis (PCA). 3,5-methoxytoluene, vanillin, coniferyl alcohol, 4-vinyl guaiacol, syringol, syringaldehyde, and guaiacol are the determining factors in the PCA analysis that are the substantially affected by COMT, CAD and CCoAOMT enzyme downregulation. COMT and CAD downregulated transgenic lines proved to be statistically different from the wild type because of a substantial difference in S and G lignin units. The sCAD line lead to a significant drop (nearly 51%) in S-lignin derived compounds, while CCoAOMT downregulation affected the least (7–11%). Further, removal of extractives via pretreatment enhanced the statistical differences among the CAD transgenic lines and its wild type. On the other hand, COMT downregulation caused 2-fold reduction in S-derived compounds compared to G-derived compounds. This study manifests the applicability of PCA analysis in tracking the biological changes in biomass (poplar in this case) and their effects on pyrolysis-oil compositions.

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