Analyzing HAAs and ketoacids without diazomethane

New methods accurately analyze HAAs and some ketoacids using an alternative methylating agent in place of the carcinogen diazomethane

Yuefeng F. Xie, David A. Reckhow, Denise C. Springborg

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Currently available methods for analyzing haloacetic acids (HAAs) and ketoacids in water generally require the use of diazomethane for sample derivatization. This article proposes methods based on a less toxic, alternative methylating agent. The reagent in both cases is acidic methanol. Side-by-side comparison of the proposed method for HAA analysis and method 6251B showed no significant difference. These two methods were also found to give similar method detection limits and spike recoveries. The new O-(2,3,4,5,6-pentafluorobenzyl)-hydroxylamine (PFBHA)-acidic methanol derivatization analytical method for two ketoacids-glyoxylic acid and pyruvic acid-was found to be comparable to the method using PFBHA-diazomethane. Additional study is needed to improve methylation efficiency for ketomalonic acid. Work is also needed to further test the proposed HAA method with monochloroacetic acid and brominated haloacetic acids.

Original languageEnglish (US)
Pages (from-to)131-138
Number of pages8
JournalJournal / American Water Works Association
Volume90
Issue number4
StatePublished - Apr 1 1998

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Diazomethane
carcinogen
Carcinogens
Hydroxylamine
Acids
acid
Methanol
Methylation
Poisons
Pyruvic Acid
methanol
Recovery
method
Water
methylation
detection method
analytical method

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Water Science and Technology

Cite this

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title = "Analyzing HAAs and ketoacids without diazomethane: New methods accurately analyze HAAs and some ketoacids using an alternative methylating agent in place of the carcinogen diazomethane",
abstract = "Currently available methods for analyzing haloacetic acids (HAAs) and ketoacids in water generally require the use of diazomethane for sample derivatization. This article proposes methods based on a less toxic, alternative methylating agent. The reagent in both cases is acidic methanol. Side-by-side comparison of the proposed method for HAA analysis and method 6251B showed no significant difference. These two methods were also found to give similar method detection limits and spike recoveries. The new O-(2,3,4,5,6-pentafluorobenzyl)-hydroxylamine (PFBHA)-acidic methanol derivatization analytical method for two ketoacids-glyoxylic acid and pyruvic acid-was found to be comparable to the method using PFBHA-diazomethane. Additional study is needed to improve methylation efficiency for ketomalonic acid. Work is also needed to further test the proposed HAA method with monochloroacetic acid and brominated haloacetic acids.",
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T2 - New methods accurately analyze HAAs and some ketoacids using an alternative methylating agent in place of the carcinogen diazomethane

AU - Xie, Yuefeng F.

AU - Reckhow, David A.

AU - Springborg, Denise C.

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N2 - Currently available methods for analyzing haloacetic acids (HAAs) and ketoacids in water generally require the use of diazomethane for sample derivatization. This article proposes methods based on a less toxic, alternative methylating agent. The reagent in both cases is acidic methanol. Side-by-side comparison of the proposed method for HAA analysis and method 6251B showed no significant difference. These two methods were also found to give similar method detection limits and spike recoveries. The new O-(2,3,4,5,6-pentafluorobenzyl)-hydroxylamine (PFBHA)-acidic methanol derivatization analytical method for two ketoacids-glyoxylic acid and pyruvic acid-was found to be comparable to the method using PFBHA-diazomethane. Additional study is needed to improve methylation efficiency for ketomalonic acid. Work is also needed to further test the proposed HAA method with monochloroacetic acid and brominated haloacetic acids.

AB - Currently available methods for analyzing haloacetic acids (HAAs) and ketoacids in water generally require the use of diazomethane for sample derivatization. This article proposes methods based on a less toxic, alternative methylating agent. The reagent in both cases is acidic methanol. Side-by-side comparison of the proposed method for HAA analysis and method 6251B showed no significant difference. These two methods were also found to give similar method detection limits and spike recoveries. The new O-(2,3,4,5,6-pentafluorobenzyl)-hydroxylamine (PFBHA)-acidic methanol derivatization analytical method for two ketoacids-glyoxylic acid and pyruvic acid-was found to be comparable to the method using PFBHA-diazomethane. Additional study is needed to improve methylation efficiency for ketomalonic acid. Work is also needed to further test the proposed HAA method with monochloroacetic acid and brominated haloacetic acids.

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