TY - JOUR
T1 - Anionic Species Regulate Chemical Storage in Nanometer Vesicles and Amperometrically Detected Exocytotic Dynamics
AU - He, Xiulan
AU - Ewing, Andrew G.
N1 - Funding Information:
The European Research Council (ERC Advanced Grant Project No. 787534 NanoBioNext), Knut and Alice Wallenberg Foundation, and the Swedish Research Council (VR Grant No. 2017-04366) are acknowledged for financial support. We would like to thank Dalsjöfors Kött AB, Sweden, and their employees for their kind help with providing the adrenal glands.
Publisher Copyright:
© 2022 The Authors. Published by American Chemical Society.
PY - 2022/3/16
Y1 - 2022/3/16
N2 - Hofmeister effects have often been ignored in living organisms, although they affect the activity and functions of biological molecules. Herein, amperometry has been applied to show that the vesicular content, dynamics of exocytosis and vesicles opening, depend on the anionic species treatment. Compared to 100 μM Cl- treated chromaffin cells, a similar number of catecholamine molecules is released after chaotropic anions (ClO4- and SCN-) treatment, even though the vesicular catecholamine content significantly increases, suggesting a lower release fraction. In addition, there are opposite effects on the dynamics of vesicles release (shorter duration) and vesicle opening (longer duration) for chaotropic anions treated cells. Our results show anion-dependent vesicle release, vesicle opening, and vesicular content, providing understanding of the pharmacological and pathological processes induced by inorganic ions.
AB - Hofmeister effects have often been ignored in living organisms, although they affect the activity and functions of biological molecules. Herein, amperometry has been applied to show that the vesicular content, dynamics of exocytosis and vesicles opening, depend on the anionic species treatment. Compared to 100 μM Cl- treated chromaffin cells, a similar number of catecholamine molecules is released after chaotropic anions (ClO4- and SCN-) treatment, even though the vesicular catecholamine content significantly increases, suggesting a lower release fraction. In addition, there are opposite effects on the dynamics of vesicles release (shorter duration) and vesicle opening (longer duration) for chaotropic anions treated cells. Our results show anion-dependent vesicle release, vesicle opening, and vesicular content, providing understanding of the pharmacological and pathological processes induced by inorganic ions.
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U2 - 10.1021/jacs.2c00581
DO - 10.1021/jacs.2c00581
M3 - Article
C2 - 35254807
AN - SCOPUS:85126312248
SN - 0002-7863
VL - 144
SP - 4310
EP - 4314
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 10
ER -