Annonaceous acetogenins: Naturally occurring inhibitors of ATP synthesis and photosystem II in spinach chloroplasts

D. Chàvez, R. Mata, Roberto Iglesias-Prieto, B. Lotina-Hennsen

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

The effects of squamocin (1), bullatacin (2) and motrilin (3), 3 bis-tetrahydrofuran Annonaceous acetogenins, isolated from Annona purpurea (Annonaceae), were investigated on several photosynthetic activities in spinach thylakoids. The results indicated that compounds 1-3 significantly inhibited both ATP synthesis and uncoupled electron transport. In addition, they enhanced light-activated Mg2+-ATPase, and basal electron flow. Therefore, acetogenins 1-3 behave as uncouplers and Hill reaction inhibitors. Natural products 1-3 did not affect photosystem I (PSI) activity but they inhibited photosystem II (PSII) electron flow. The study of the partial PSII reactions from H2O to DCPIPox, H2O to SiMo and diphenylcarbazide to DCPIP established that the site of inhibition was at the oxygen-evolving complex (OEC). Chlorophyll a fluorescence measurements confirmed the behavior of the Annonaceous acetogenins as water-splitting enzyme inhibitors.

Original languageEnglish (US)
Pages (from-to)262-268
Number of pages7
JournalPhysiologia Plantarum
Volume111
Issue number2
DOIs
StatePublished - Nov 7 2001

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

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