Anodic biofilms in microbial fuel cells harbor low numbers of higher-power-producing bacteria than abundant genera

Patrick D. Kiely, Douglas F. Call, Matthew D. Yates, John Ragan, Bruce Ernest Logan

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Microbial fuel cell (MFC) anode communities often reveal just a few genera, but it is not known to what extent less abundant bacteria could be important for improving performance. We examined the microbial community in an MFC fed with formic acid for more than 1 year and determined using 16S rRNA gene cloning and fluorescent in situ hybridization that members of the Paracoccus genus comprised most (~30%) of the anode community. A Paracoccus isolate obtained from this biofilm (Paracoccus denitrificans strain PS-1) produced only 5.6 mW/m 2, whereas the original mixed culture produced up to 10 mW/m 2. Despite the absence of any Shewanella species in the clone library, we isolated a strain of Shewanella putrefaciens (strain PS-2) from the same biofilm capable of producing a higher-power density (17.4 mW/m2) than the mixed culture, although voltage generation was variable. Our results suggest that the numerical abundance of microorganisms in biofilms cannot be assumed a priori to correlate to capacities of these predominant species for high-power production. Detailed screening of bacterial biofilms may therefore be needed to identify important strains capable of high-power generation for specific substrates.

Original languageEnglish (US)
Pages (from-to)371-380
Number of pages10
JournalApplied Microbiology and Biotechnology
Volume88
Issue number1
DOIs
StatePublished - Sep 1 2010

Fingerprint

Bioelectric Energy Sources
Biofilms
Paracoccus
Bacteria
formic acid
Electrodes
Shewanella putrefaciens
Paracoccus denitrificans
Shewanella
Fluorescence In Situ Hybridization
rRNA Genes
Organism Cloning
Clone Cells

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

@article{f30bd69a8b0a4b0ab6df9f8ebb13d391,
title = "Anodic biofilms in microbial fuel cells harbor low numbers of higher-power-producing bacteria than abundant genera",
abstract = "Microbial fuel cell (MFC) anode communities often reveal just a few genera, but it is not known to what extent less abundant bacteria could be important for improving performance. We examined the microbial community in an MFC fed with formic acid for more than 1 year and determined using 16S rRNA gene cloning and fluorescent in situ hybridization that members of the Paracoccus genus comprised most (~30{\%}) of the anode community. A Paracoccus isolate obtained from this biofilm (Paracoccus denitrificans strain PS-1) produced only 5.6 mW/m 2, whereas the original mixed culture produced up to 10 mW/m 2. Despite the absence of any Shewanella species in the clone library, we isolated a strain of Shewanella putrefaciens (strain PS-2) from the same biofilm capable of producing a higher-power density (17.4 mW/m2) than the mixed culture, although voltage generation was variable. Our results suggest that the numerical abundance of microorganisms in biofilms cannot be assumed a priori to correlate to capacities of these predominant species for high-power production. Detailed screening of bacterial biofilms may therefore be needed to identify important strains capable of high-power generation for specific substrates.",
author = "Kiely, {Patrick D.} and Call, {Douglas F.} and Yates, {Matthew D.} and John Ragan and Logan, {Bruce Ernest}",
year = "2010",
month = "9",
day = "1",
doi = "10.1007/s00253-010-2757-2",
language = "English (US)",
volume = "88",
pages = "371--380",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer Verlag",
number = "1",

}

Anodic biofilms in microbial fuel cells harbor low numbers of higher-power-producing bacteria than abundant genera. / Kiely, Patrick D.; Call, Douglas F.; Yates, Matthew D.; Ragan, John; Logan, Bruce Ernest.

In: Applied Microbiology and Biotechnology, Vol. 88, No. 1, 01.09.2010, p. 371-380.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Anodic biofilms in microbial fuel cells harbor low numbers of higher-power-producing bacteria than abundant genera

AU - Kiely, Patrick D.

AU - Call, Douglas F.

AU - Yates, Matthew D.

AU - Ragan, John

AU - Logan, Bruce Ernest

PY - 2010/9/1

Y1 - 2010/9/1

N2 - Microbial fuel cell (MFC) anode communities often reveal just a few genera, but it is not known to what extent less abundant bacteria could be important for improving performance. We examined the microbial community in an MFC fed with formic acid for more than 1 year and determined using 16S rRNA gene cloning and fluorescent in situ hybridization that members of the Paracoccus genus comprised most (~30%) of the anode community. A Paracoccus isolate obtained from this biofilm (Paracoccus denitrificans strain PS-1) produced only 5.6 mW/m 2, whereas the original mixed culture produced up to 10 mW/m 2. Despite the absence of any Shewanella species in the clone library, we isolated a strain of Shewanella putrefaciens (strain PS-2) from the same biofilm capable of producing a higher-power density (17.4 mW/m2) than the mixed culture, although voltage generation was variable. Our results suggest that the numerical abundance of microorganisms in biofilms cannot be assumed a priori to correlate to capacities of these predominant species for high-power production. Detailed screening of bacterial biofilms may therefore be needed to identify important strains capable of high-power generation for specific substrates.

AB - Microbial fuel cell (MFC) anode communities often reveal just a few genera, but it is not known to what extent less abundant bacteria could be important for improving performance. We examined the microbial community in an MFC fed with formic acid for more than 1 year and determined using 16S rRNA gene cloning and fluorescent in situ hybridization that members of the Paracoccus genus comprised most (~30%) of the anode community. A Paracoccus isolate obtained from this biofilm (Paracoccus denitrificans strain PS-1) produced only 5.6 mW/m 2, whereas the original mixed culture produced up to 10 mW/m 2. Despite the absence of any Shewanella species in the clone library, we isolated a strain of Shewanella putrefaciens (strain PS-2) from the same biofilm capable of producing a higher-power density (17.4 mW/m2) than the mixed culture, although voltage generation was variable. Our results suggest that the numerical abundance of microorganisms in biofilms cannot be assumed a priori to correlate to capacities of these predominant species for high-power production. Detailed screening of bacterial biofilms may therefore be needed to identify important strains capable of high-power generation for specific substrates.

UR - http://www.scopus.com/inward/record.url?scp=77955851595&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955851595&partnerID=8YFLogxK

U2 - 10.1007/s00253-010-2757-2

DO - 10.1007/s00253-010-2757-2

M3 - Article

C2 - 20632002

AN - SCOPUS:77955851595

VL - 88

SP - 371

EP - 380

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 1

ER -