Anti-fungal activity of maize silk proteins and role of chitinases in Aspergillus flavus resistance

Bela Peethambaran, Leigh Hawkins, Gary L. Windham, W. Paul Williams, Dawn S. Luthe

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Studies were conducted to identify proteins in maize silks that may be contributing to Aspergillus flavus resistance. We first performed bioassays using silk extracts collected from two A. flavus-resistant inbred lines and two susceptible inbred lines. Fungal biomass was quantified by measuring fluorescence of a green fluorescent protein (GFP)-tagged A. flavus and by measuring ergosterol levels. The silk extracts from resistant inbreds had greater anti-fungal activity compared to susceptible inbreds. Comparative proteomic analysis of the two resistant and susceptible inbreds led to the identification of several anti-fungal proteins. One of the anti-fungal proteins that we further investigated was chitinase. There were three chitinases that were differentially expressed in the resistant lines (PRm3 chitinase, chitinase I, and chitinase A). We conducted chitinase assays on silk proteins from extracts of resistant and susceptible inbred lines. Silk extracts from resistant inbred lines showed significantly higher activity in the resistant maize inbreds compared to the susceptible inbreds (P<0.01). The differential expression of chitinases in maize resistant and susceptible inbred silks suggests that these proteins may contribute to A. flavus resistance.

Original languageEnglish (US)
Pages (from-to)27-39
Number of pages13
JournalToxin Reviews
Volume29
Issue number1
DOIs
StatePublished - Mar 1 2010

Fingerprint

Aspergillus flavus
Chitinases
Silk
Aspergillus
Zea mays
Proteins
Fungal Proteins
Ergosterol
Bioassay
Green Fluorescent Proteins
Biological Assay
Proteomics
Biomass
Assays
Fluorescence

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Peethambaran, B., Hawkins, L., Windham, G. L., Williams, W. P., & Luthe, D. S. (2010). Anti-fungal activity of maize silk proteins and role of chitinases in Aspergillus flavus resistance. Toxin Reviews, 29(1), 27-39. https://doi.org/10.3109/15569540903402874
Peethambaran, Bela ; Hawkins, Leigh ; Windham, Gary L. ; Williams, W. Paul ; Luthe, Dawn S. / Anti-fungal activity of maize silk proteins and role of chitinases in Aspergillus flavus resistance. In: Toxin Reviews. 2010 ; Vol. 29, No. 1. pp. 27-39.
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Peethambaran, B, Hawkins, L, Windham, GL, Williams, WP & Luthe, DS 2010, 'Anti-fungal activity of maize silk proteins and role of chitinases in Aspergillus flavus resistance', Toxin Reviews, vol. 29, no. 1, pp. 27-39. https://doi.org/10.3109/15569540903402874

Anti-fungal activity of maize silk proteins and role of chitinases in Aspergillus flavus resistance. / Peethambaran, Bela; Hawkins, Leigh; Windham, Gary L.; Williams, W. Paul; Luthe, Dawn S.

In: Toxin Reviews, Vol. 29, No. 1, 01.03.2010, p. 27-39.

Research output: Contribution to journalArticle

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