Anti-inflammatory effects of polyunsaturated fatty acids in THP-1 cells

Guixiang Zhao, Terry D. Etherton, Keith R. Martin, John P. Vanden Heuvel, Peter J. Gillies, Sheila G. West, Penny M. Kris-Etherton

Research output: Contribution to journalArticlepeer-review

222 Scopus citations

Abstract

The effects of linoleic acid (LA), α-linolenic acid (ALA), and docosahexaenoic acid (DHA) were compared to that of palmitic acid (PA), on inflammatory responses in human monocytic THP-1 cells. When cells were pre-incubated with fatty acids for 2-h and then stimulated with lipopolysaccharide for 24-h in the presence of fatty acids, secretion of interleukin (IL)-6, IL-1β, and tumor necrosis factor-α (TNFα) was significantly decreased after treatment with LA, ALA, and DHA versus PA (P < 0.01 for all); ALA and DHA elicited more favorable effects. These effects were comparable to those for 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) and were dose-dependent. In addition, LA, ALA, and DHA decreased IL-6, IL-1β, and TNFα gene expression (P < 0.05 for all) and nuclear factor (NF)-κB DNA-binding activity, whereas peroxisome proliferator-activated receptor-γ (PPARγ) DNA-binding activity was increased. The results indicate that the anti-inflammatory effects of polyunsaturated fatty acids may be, in part, due to the inhibition of NF-κB activation via activation of PPARγ.

Original languageEnglish (US)
Pages (from-to)909-917
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume336
Issue number3
DOIs
StatePublished - Oct 28 2005

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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