Antibodies to distinct polypeptides of RNA polymerase I in sera from patients with rheumatic autoimmune disease

D. A. Stetler, K. M. Rose, M. E. Wenger, C. M. Berlin, S. T. Jacob

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Abstract

Sera from patients with rheumatic autoimmune diseases were screened for antibodies directed against RNA polymerase I by using a solid-phase radioimmunoassay. Significant levels of the antibodies were detected in the sera of all patients with either systemic lupus erythematosus or mixed connective tissue disease and in 78% of the individuals with rheumatoid arthritis. No detectable anti-RNA polymerase I antibodies were found in the sera from healthy subjects. Individuals taking hydralazine, three of whom exhibited symptoms of drug-induced lupus, had barely detectable levels of the antibodies. Immunoglobulins obtained from sera containing anti-RNA polymerase I antibodies, as determined by the radioimmunoassay, could inhibit RNA polymerase I activity in vitro. Sera from patients with systemic lupus erythematosus contained immunoglobulins directed against the polymerase I-associated polypeptide of M[r] 65,000 as well as against the polypeptides of M[r] 120,000 or M[r] 25,000, or both. Sera from individuals with rheumatoid arthritis reacted with the polypeptide of M[r] 65,000 only. The antibodies in the sera of patients with mixed connective tissue disease were directed against the M[r] 42,000 polypeptide or a combination of the M[r] 65,000, 42,000, and 25,000 polypeptides. These data suggest that the production of anti-RNA polymerase I antibodies may be a unique characteristic of individuals with rheumatic autoimmune diseases and that the production of antibodies against specific polypeptides of RNA polymerase I may be indicative of the particular class of disease.

Original languageEnglish (US)
Pages (from-to)7499-7503
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume79
Issue number23 I
DOIs
Publication statusPublished - Dec 1 1982

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All Science Journal Classification (ASJC) codes

  • General

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