Antibody-mediated bacterial clearance from the lower respiratory tract of mice requires complement component C3

Elizabeth J. Pishko, Girish Soorapp Kirimanjeswara, Mylisa R. Pilione, Lakshmi Gopinathan, Mary J. Kennett, Eric Thomas Harvill

Research output: Contribution to journalArticle

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Abstract

To assess the contribution of complement to respiratory immunity in the context of a natural bacterial infection, we used mice genetically deficient in complement components and the murine pathogen Bordetella bronchiseptica. Complement component C3 was not required for the control of bacterial infection or for the generation of infection-induced protective immunity. However, C3-deficient (C3-/-) mice were severely defective, compared to wild type, in vaccine-induced protective immunity. Adoptively transferred immune serum from convalescent wild-type or C3-/- animals rapidly cleared B. bronchiseptica from the lungs of wild-type mice but did not affect its growth in C3-/- mice, indicating that the defect is not in the generation of protective immunity, but in its function. Immune serum was effective in C5-deficient mice but had little effect in the lungs of mice lacking either Fcγ receptors (FcγR) or CR3, suggesting bacterial clearance is not via direct complement-mediated lysis. Together, these data indicate that complement is required for antibody-mediated clearance of Bordetella and suggest the mechanism involves C3 opsonization of bacteria for phagocytosis that is both CR3- and FcγR-dependent.

Original languageEnglish (US)
Pages (from-to)184-193
Number of pages10
JournalEuropean Journal of Immunology
Volume34
Issue number1
DOIs
StatePublished - Jan 1 2004

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Bacterial Antibodies
Complement C3
Respiratory System
Immunity
Bordetella bronchiseptica
Fc Receptors
Bacterial Infections
Immune Sera
Bordetella
Macrophage-1 Antigen
Lung
Phagocytosis
Vaccines
Bacteria
Antibodies
Growth
Infection

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Pishko, Elizabeth J. ; Kirimanjeswara, Girish Soorapp ; Pilione, Mylisa R. ; Gopinathan, Lakshmi ; Kennett, Mary J. ; Harvill, Eric Thomas. / Antibody-mediated bacterial clearance from the lower respiratory tract of mice requires complement component C3. In: European Journal of Immunology. 2004 ; Vol. 34, No. 1. pp. 184-193.
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Antibody-mediated bacterial clearance from the lower respiratory tract of mice requires complement component C3. / Pishko, Elizabeth J.; Kirimanjeswara, Girish Soorapp; Pilione, Mylisa R.; Gopinathan, Lakshmi; Kennett, Mary J.; Harvill, Eric Thomas.

In: European Journal of Immunology, Vol. 34, No. 1, 01.01.2004, p. 184-193.

Research output: Contribution to journalArticle

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