TY - JOUR
T1 - Antigen expression during murine γ-herpesvirus infection
AU - Woodland, David L.
AU - Flaño, Emilio
AU - Usherwood, Edward J.
AU - Liu, Luzheng
AU - Kim, In Jeong
AU - Husain, S. Mazher
AU - Sample, Jeffery T.
AU - Blackman, Marcia A.
N1 - Funding Information:
We thank SCOTTIE ADAMS and TIM MILLER of the Trudeau Institute Molecular Biology Core Facility for the generation of tetrameric reagents. This work was supported by NIH grants AI37597 (D. L. W.), AI42927 (M. A. B.), CA56639 (J. T. S.), the American Lebanese Syrian Associated Charities, and the Trudeau Institute.
PY - 2001
Y1 - 2001
N2 - Gammaherpesviruses (γHV) establish a life-long latency in the host and are associated with a number of malignant human diseases. It is generally believed that T cells play a major role in controlling the initial acute infection and subsequently maintaining the virus in a quiescent state. However, the nature of the T cell response to γ-herpesvirus infections is poorly understood. In the current report we took advantage of a mouse model of γHV infection (murine herpesvirus-68, MHV-68) to investigate the T cell response to different phases of the infection. Intranasal infection with MHV-68 induces an acute infection in lung epithelial cells and long-term latency in B cells. The kinetics of the CD8+ T cell response to different lytic cycle and latency-associated antigens was highly complex and distinct patterns of response could be identified. These responses were regulated by multiple factors including differences in temporal expression of the relevant antigens, differences in the presentation of antigen in different organs, and differential expression of antigen in different types of antigen presenting cells. For example, some antigens were expressed at distinct phases of the infection and in specific organs or subsets of antigen presenting cells. In addition, recent data suggest that in addition to B cells, both macrophages and dendritic cells harbor latent MHV-68 infection, adding further complexity to their role in controlling the T cell response to this infection.
AB - Gammaherpesviruses (γHV) establish a life-long latency in the host and are associated with a number of malignant human diseases. It is generally believed that T cells play a major role in controlling the initial acute infection and subsequently maintaining the virus in a quiescent state. However, the nature of the T cell response to γ-herpesvirus infections is poorly understood. In the current report we took advantage of a mouse model of γHV infection (murine herpesvirus-68, MHV-68) to investigate the T cell response to different phases of the infection. Intranasal infection with MHV-68 induces an acute infection in lung epithelial cells and long-term latency in B cells. The kinetics of the CD8+ T cell response to different lytic cycle and latency-associated antigens was highly complex and distinct patterns of response could be identified. These responses were regulated by multiple factors including differences in temporal expression of the relevant antigens, differences in the presentation of antigen in different organs, and differential expression of antigen in different types of antigen presenting cells. For example, some antigens were expressed at distinct phases of the infection and in specific organs or subsets of antigen presenting cells. In addition, recent data suggest that in addition to B cells, both macrophages and dendritic cells harbor latent MHV-68 infection, adding further complexity to their role in controlling the T cell response to this infection.
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U2 - 10.1078/0171-2985-00104
DO - 10.1078/0171-2985-00104
M3 - Article
C2 - 11846230
AN - SCOPUS:0035713151
VL - 204
SP - 649
EP - 658
JO - Zeitschrift für Immunitätsforschung und experimentelle Therapie
JF - Zeitschrift für Immunitätsforschung und experimentelle Therapie
SN - 0171-2985
IS - 5
ER -