Antimicrobial activity of a porcine myeloperoxidase against plant pathogenic bacteria and fungi

Yinong Yang, Edwin J. Anderson

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Porcine myeloperoxidase was evaluated for its antimicrobial activity against plant pathogenic bacteria and fungi. The results indicated that the enzyme, in the presence of a small amount of hydrogen peroxide, was effective against a broad spectrum of plant pathogens. The growth of seven bacterial species, including nine pathovars, from the genera Erwinia, Pseudomonas and Xanthomonas, was significantly inhibited by the enzyme at a concentration as low as 0.4 U ml-1 , while 4-0 U ml-1 was lethal to all plant pathogenic bacteria examined. Myeloperoxidase, at 40 U ml-1, was lethal to germinating spores from three isolates of the fungal plant pathogen Fusarium solani and two isolates from each of Colletotrichum gloeosporioides and C. malvarum. The enzyme's antifungal effects on the rice blast pathogen Magnaporthe grisea were studied both in vitro and on host plants. The enzyme significantly inhibited spore germination of two isolates of M. grisea races IC17 and IB49 at concentrations over 16 U ml-1, and disintegration of fungal spore walls was caused by 80 U ml-1. The enzyme was even more effective in reducing disease incidence of blast on young rice plants treated with 0.5 U ml-1, while 2-5 U ml-1 resulted in complete inhibition of infection. These results support and further extend the suggestion that myeloperoxidase could be used as a broad-spectrum biocontrol agent or as a transgenically expressed protein to combat diseases caused by plant pathogenic bacteria and fungi.

Original languageEnglish (US)
Pages (from-to)211-220
Number of pages10
JournalJournal of Applied Microbiology
Volume86
Issue number2
DOIs
StatePublished - Jan 1 1999

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Peroxidase
Fungi
Swine
Bacteria
Magnaporthe
Enzymes
Spores
Erwinia
Xanthomonas
Colletotrichum
Plant Diseases
Fungal Spores
Fusarium
Germination
Pseudomonas
Hydrogen Peroxide
Incidence
Growth
Infection
Proteins

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

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title = "Antimicrobial activity of a porcine myeloperoxidase against plant pathogenic bacteria and fungi",
abstract = "Porcine myeloperoxidase was evaluated for its antimicrobial activity against plant pathogenic bacteria and fungi. The results indicated that the enzyme, in the presence of a small amount of hydrogen peroxide, was effective against a broad spectrum of plant pathogens. The growth of seven bacterial species, including nine pathovars, from the genera Erwinia, Pseudomonas and Xanthomonas, was significantly inhibited by the enzyme at a concentration as low as 0.4 U ml-1 , while 4-0 U ml-1 was lethal to all plant pathogenic bacteria examined. Myeloperoxidase, at 40 U ml-1, was lethal to germinating spores from three isolates of the fungal plant pathogen Fusarium solani and two isolates from each of Colletotrichum gloeosporioides and C. malvarum. The enzyme's antifungal effects on the rice blast pathogen Magnaporthe grisea were studied both in vitro and on host plants. The enzyme significantly inhibited spore germination of two isolates of M. grisea races IC17 and IB49 at concentrations over 16 U ml-1, and disintegration of fungal spore walls was caused by 80 U ml-1. The enzyme was even more effective in reducing disease incidence of blast on young rice plants treated with 0.5 U ml-1, while 2-5 U ml-1 resulted in complete inhibition of infection. These results support and further extend the suggestion that myeloperoxidase could be used as a broad-spectrum biocontrol agent or as a transgenically expressed protein to combat diseases caused by plant pathogenic bacteria and fungi.",
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Antimicrobial activity of a porcine myeloperoxidase against plant pathogenic bacteria and fungi. / Yang, Yinong; Anderson, Edwin J.

In: Journal of Applied Microbiology, Vol. 86, No. 2, 01.01.1999, p. 211-220.

Research output: Contribution to journalArticle

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