Antimicrobial activity of seaweeds extracts against multiresistant pathogens

Shanmughapriya Santhanam, Aseer Manilal, Sugathan Sujith, Joseph Selvin, George Seghal Kiran, Kalimuthusamy Natarajaseenivasan

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Fourteen seaweeds collected from the intertidal zone of Southwest coast of India were tested against ten human pathogen bacteria and one human pathogen fungus using the well diffusion test in the casitone agar medium. The species used in the present study include five Chlorophyta (Bryopsis plumosa, Ulva fasciata, Acrosiphonia orientalis, Chaetomorpha antennina, Grateloupia filicina), five Rhodophyta (Hypnea pannosa, Gracilaria corticata, Centroceras clavulatum, Portieria hornemannii, Cheilosporum spectabile) and four Phaeophyta (Padina tetrastromatica, Sargassum wightii, Stocheospermum marginatum, Chnoospora bicanaliculata). Of these, seven species were determined to be highly bioactive and screened on the multiresistant pathogens. We found that drying process has eliminated the active principles in the seaweeds. In the present study, methanoktoluene (3:1) was found to be the best solvent for extracting the antimicrobial principles from fresh algae. However, the ethanolic extract showed no antibacterial activity. Acrosiphonia orientalis showed activity against 70% of the tested organisms. Stocheospermum marginatum was the only seaweed that showed activity against Klebsiella pneumoniae. The extract from Gracilaria corticata was highly active against Proteus mirabilis, a Gram negative pathogenic bacterium. The present findings revealed that the tested seaweeds were highly active against Gram negative bacteria than Gram positive bacteria. The antimicrobial principle from seaweed was found to be a lipophilic compound. The compound was stable over a wide range of temperature (30-60 °C). The active principles of highly active seaweeds Acrosiphonia orientalis and Stocheospermum marginatum were bactericidal.

Original languageEnglish (US)
Pages (from-to)535-541
Number of pages7
JournalAnnals of Microbiology
Volume58
Issue number3
DOIs
StatePublished - Jan 1 2008

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Seaweed
Gracilaria
Gram-Negative Bacteria
Sargassum
Ulva
Phaeophyta
Rhodophyta
Proteus mirabilis
Chlorophyta
Klebsiella pneumoniae
Gram-Positive Bacteria
Agar
India
Fungi
Bacteria
Temperature

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology

Cite this

Santhanam, S., Manilal, A., Sujith, S., Selvin, J., Kiran, G. S., & Natarajaseenivasan, K. (2008). Antimicrobial activity of seaweeds extracts against multiresistant pathogens. Annals of Microbiology, 58(3), 535-541. https://doi.org/10.1007/BF03175554
Santhanam, Shanmughapriya ; Manilal, Aseer ; Sujith, Sugathan ; Selvin, Joseph ; Kiran, George Seghal ; Natarajaseenivasan, Kalimuthusamy. / Antimicrobial activity of seaweeds extracts against multiresistant pathogens. In: Annals of Microbiology. 2008 ; Vol. 58, No. 3. pp. 535-541.
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Santhanam, S, Manilal, A, Sujith, S, Selvin, J, Kiran, GS & Natarajaseenivasan, K 2008, 'Antimicrobial activity of seaweeds extracts against multiresistant pathogens', Annals of Microbiology, vol. 58, no. 3, pp. 535-541. https://doi.org/10.1007/BF03175554

Antimicrobial activity of seaweeds extracts against multiresistant pathogens. / Santhanam, Shanmughapriya; Manilal, Aseer; Sujith, Sugathan; Selvin, Joseph; Kiran, George Seghal; Natarajaseenivasan, Kalimuthusamy.

In: Annals of Microbiology, Vol. 58, No. 3, 01.01.2008, p. 535-541.

Research output: Contribution to journalArticle

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