Antimicrobial resistance determinants in acinetobacter baumannii isolates taken from military treatment facilities

Chris Rowe Taitt, Tomasz A. Leski, Michael G. Stockelman, David W. Craft, Daniel V. Zurawski, Benjamin C. Kirkup, Gary J. Vora

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Multidrug-resistant (MDR) Acinetobacter baumannii infections are of particular concern within medical treatment facilities, yet the gene assemblages that give rise to this phenotype remain poorly characterized. In this study, we tested 97 clinical A. baumannii isolates collected from military treatment facilities (MTFs) from 2003 to 2009 by using a molecular epidemiological approach that enabled for the simultaneous screening of 236 antimicrobial resistance genes. Overall, 80% of the isolates were found to be MDR, each strain harbored between one and 17 resistant determinants, and a total of 52 unique resistance determinants or gene families were detected which are known to confer resistance to β-lactam (e.g., blaGES-11, blaTEM, blaOXA-58), aminoglycoside (e.g., aphA1, aacC1, armA), macrolide (msrA, msrB), tetracycline [e.g., tet(A), tet(B), tet(39)], phenicol (e.g., cmlA4, catA1, cat4), quaternary amine (qacE, qacE-1), streptothricin (sat2), sulfonamide (sul1, sul2), and diaminopyrimidine (dfrA1, dfrA7, dfrA19) antimicrobial compounds. Importantly, 91% of the isolates harbored blaOXA-51-like carbapenemase genes (including six new variants), 40% harbored the blaOXA-23 carbapenemase gene, and 89% contained a variety of aminoglycoside resistance determinants with up to six unique determinants identified per strain. Many of the resistance determinants were found in potentially mobile gene cassettes; 45% and 7% of the isolates contained class 1 and class 2 integrons, respectively. Combined, the results demonstrate a facile approach that supports a more complete understanding of the genetic underpinnings of antimicrobial resistance to better assess the load, transmission, and evolution of MDR in MTF-associated A. baumannii.

Original languageEnglish (US)
Pages (from-to)767-781
Number of pages15
JournalAntimicrobial agents and chemotherapy
Volume58
Issue number2
DOIs
StatePublished - Feb 1 2014

Fingerprint

Military Facilities
Acinetobacter baumannii
Genes
Aminoglycosides
Streptothricins
Acinetobacter Infections
Integrons
Lactams
Sulfonamides
Macrolides
Tetracycline
Amines
Phenotype

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Taitt, Chris Rowe ; Leski, Tomasz A. ; Stockelman, Michael G. ; Craft, David W. ; Zurawski, Daniel V. ; Kirkup, Benjamin C. ; Vora, Gary J. / Antimicrobial resistance determinants in acinetobacter baumannii isolates taken from military treatment facilities. In: Antimicrobial agents and chemotherapy. 2014 ; Vol. 58, No. 2. pp. 767-781.
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abstract = "Multidrug-resistant (MDR) Acinetobacter baumannii infections are of particular concern within medical treatment facilities, yet the gene assemblages that give rise to this phenotype remain poorly characterized. In this study, we tested 97 clinical A. baumannii isolates collected from military treatment facilities (MTFs) from 2003 to 2009 by using a molecular epidemiological approach that enabled for the simultaneous screening of 236 antimicrobial resistance genes. Overall, 80{\%} of the isolates were found to be MDR, each strain harbored between one and 17 resistant determinants, and a total of 52 unique resistance determinants or gene families were detected which are known to confer resistance to β-lactam (e.g., blaGES-11, blaTEM, blaOXA-58), aminoglycoside (e.g., aphA1, aacC1, armA), macrolide (msrA, msrB), tetracycline [e.g., tet(A), tet(B), tet(39)], phenicol (e.g., cmlA4, catA1, cat4), quaternary amine (qacE, qacE-1), streptothricin (sat2), sulfonamide (sul1, sul2), and diaminopyrimidine (dfrA1, dfrA7, dfrA19) antimicrobial compounds. Importantly, 91{\%} of the isolates harbored blaOXA-51-like carbapenemase genes (including six new variants), 40{\%} harbored the blaOXA-23 carbapenemase gene, and 89{\%} contained a variety of aminoglycoside resistance determinants with up to six unique determinants identified per strain. Many of the resistance determinants were found in potentially mobile gene cassettes; 45{\%} and 7{\%} of the isolates contained class 1 and class 2 integrons, respectively. Combined, the results demonstrate a facile approach that supports a more complete understanding of the genetic underpinnings of antimicrobial resistance to better assess the load, transmission, and evolution of MDR in MTF-associated A. baumannii.",
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Antimicrobial resistance determinants in acinetobacter baumannii isolates taken from military treatment facilities. / Taitt, Chris Rowe; Leski, Tomasz A.; Stockelman, Michael G.; Craft, David W.; Zurawski, Daniel V.; Kirkup, Benjamin C.; Vora, Gary J.

In: Antimicrobial agents and chemotherapy, Vol. 58, No. 2, 01.02.2014, p. 767-781.

Research output: Contribution to journalArticle

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