Antimicrobial resistance of Escherichia coli O157 isolated from humans, cattle, swine, and food

Carl M. Schroeder, Cuiwei Zhao, Chitrita DebRoy, Jocelyn Torcolini, Shaohua Zhao, David G. White, David D. Wagner, Patrick F. McDermott, Robert D. Walker, Jianghong Meng

Research output: Contribution to journalArticle

190 Citations (Scopus)

Abstract

A total of 361 Escherichia coli O157 isolates, recovered from humans, cattle, swine, and food during the years 1985 to 2000, were examined to better understand the prevalence of antimicrobial resistance among these organisms. Based on broth microdilution results, 220 (61%) of the isolates were susceptible to all 13 antimicrobials tested. Ninety-nine (27%) of the isolates, however, were resistant to tetracycline, 93 (26%) were resistant to sulfamethoxazole, 61 (17%) were resistant to cephalothin, and 48 (13%) were resistant to ampicillin. Highest frequencies of resistance occurred among swine isolates (n = 70), where 52 (74%) were resistant to sulfamethoxazole, 50 (71%) were resistant to tetracycline, 38 (54%) were resistant to cephalothin, and 17 (24%) were resistant to ampicillin. Based on the presence of Shiga toxin genes as determined by PCR, 210 (58%) of the isolates were identified as Shiga toxin-producing E. coli (STEC). Among these, resistance was generally low, yet 21 (10%) were resistant to sulfamethoxazole and 19 (9%) were resistant to tetracycline. Based on latex agglutination, 189 (52%) of the isolates were identified as E. coli O157:H7, among which 19 (10%) were resistant to sulfamethoxazole and 16 (8%) were resistant to tetracycline. The data suggest that selection pressure imposed by the use of tetracycline derivatives, sulfa drugs, cephalosporins, and penicillins, whether therapeutically in human and veterinary medicine or as prophylaxis in the animal production environment, is a key driving force in the selection of antimicrobial resistance in STEC and non-STEC O157.

Original languageEnglish (US)
Pages (from-to)576-581
Number of pages6
JournalApplied and environmental microbiology
Volume68
Issue number2
DOIs
StatePublished - Feb 11 2002

Fingerprint

Escherichia coli O157
Sulfamethoxazole
Tetracycline
tetracycline
sulfamethoxazole
antibiotic resistance
toxin
cattle
Swine
Food
swine
food
Shiga-Toxigenic Escherichia coli
cephalothin
Cephalothin
Shiga toxin-producing Escherichia coli
Ampicillin
ampicillin
Shiga Toxin
Shiga toxin

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Schroeder, C. M., Zhao, C., DebRoy, C., Torcolini, J., Zhao, S., White, D. G., ... Meng, J. (2002). Antimicrobial resistance of Escherichia coli O157 isolated from humans, cattle, swine, and food. Applied and environmental microbiology, 68(2), 576-581. https://doi.org/10.1128/AEM.68.2.576-581.2002
Schroeder, Carl M. ; Zhao, Cuiwei ; DebRoy, Chitrita ; Torcolini, Jocelyn ; Zhao, Shaohua ; White, David G. ; Wagner, David D. ; McDermott, Patrick F. ; Walker, Robert D. ; Meng, Jianghong. / Antimicrobial resistance of Escherichia coli O157 isolated from humans, cattle, swine, and food. In: Applied and environmental microbiology. 2002 ; Vol. 68, No. 2. pp. 576-581.
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Schroeder, CM, Zhao, C, DebRoy, C, Torcolini, J, Zhao, S, White, DG, Wagner, DD, McDermott, PF, Walker, RD & Meng, J 2002, 'Antimicrobial resistance of Escherichia coli O157 isolated from humans, cattle, swine, and food', Applied and environmental microbiology, vol. 68, no. 2, pp. 576-581. https://doi.org/10.1128/AEM.68.2.576-581.2002

Antimicrobial resistance of Escherichia coli O157 isolated from humans, cattle, swine, and food. / Schroeder, Carl M.; Zhao, Cuiwei; DebRoy, Chitrita; Torcolini, Jocelyn; Zhao, Shaohua; White, David G.; Wagner, David D.; McDermott, Patrick F.; Walker, Robert D.; Meng, Jianghong.

In: Applied and environmental microbiology, Vol. 68, No. 2, 11.02.2002, p. 576-581.

Research output: Contribution to journalArticle

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T1 - Antimicrobial resistance of Escherichia coli O157 isolated from humans, cattle, swine, and food

AU - Schroeder, Carl M.

AU - Zhao, Cuiwei

AU - DebRoy, Chitrita

AU - Torcolini, Jocelyn

AU - Zhao, Shaohua

AU - White, David G.

AU - Wagner, David D.

AU - McDermott, Patrick F.

AU - Walker, Robert D.

AU - Meng, Jianghong

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Y1 - 2002/2/11

N2 - A total of 361 Escherichia coli O157 isolates, recovered from humans, cattle, swine, and food during the years 1985 to 2000, were examined to better understand the prevalence of antimicrobial resistance among these organisms. Based on broth microdilution results, 220 (61%) of the isolates were susceptible to all 13 antimicrobials tested. Ninety-nine (27%) of the isolates, however, were resistant to tetracycline, 93 (26%) were resistant to sulfamethoxazole, 61 (17%) were resistant to cephalothin, and 48 (13%) were resistant to ampicillin. Highest frequencies of resistance occurred among swine isolates (n = 70), where 52 (74%) were resistant to sulfamethoxazole, 50 (71%) were resistant to tetracycline, 38 (54%) were resistant to cephalothin, and 17 (24%) were resistant to ampicillin. Based on the presence of Shiga toxin genes as determined by PCR, 210 (58%) of the isolates were identified as Shiga toxin-producing E. coli (STEC). Among these, resistance was generally low, yet 21 (10%) were resistant to sulfamethoxazole and 19 (9%) were resistant to tetracycline. Based on latex agglutination, 189 (52%) of the isolates were identified as E. coli O157:H7, among which 19 (10%) were resistant to sulfamethoxazole and 16 (8%) were resistant to tetracycline. The data suggest that selection pressure imposed by the use of tetracycline derivatives, sulfa drugs, cephalosporins, and penicillins, whether therapeutically in human and veterinary medicine or as prophylaxis in the animal production environment, is a key driving force in the selection of antimicrobial resistance in STEC and non-STEC O157.

AB - A total of 361 Escherichia coli O157 isolates, recovered from humans, cattle, swine, and food during the years 1985 to 2000, were examined to better understand the prevalence of antimicrobial resistance among these organisms. Based on broth microdilution results, 220 (61%) of the isolates were susceptible to all 13 antimicrobials tested. Ninety-nine (27%) of the isolates, however, were resistant to tetracycline, 93 (26%) were resistant to sulfamethoxazole, 61 (17%) were resistant to cephalothin, and 48 (13%) were resistant to ampicillin. Highest frequencies of resistance occurred among swine isolates (n = 70), where 52 (74%) were resistant to sulfamethoxazole, 50 (71%) were resistant to tetracycline, 38 (54%) were resistant to cephalothin, and 17 (24%) were resistant to ampicillin. Based on the presence of Shiga toxin genes as determined by PCR, 210 (58%) of the isolates were identified as Shiga toxin-producing E. coli (STEC). Among these, resistance was generally low, yet 21 (10%) were resistant to sulfamethoxazole and 19 (9%) were resistant to tetracycline. Based on latex agglutination, 189 (52%) of the isolates were identified as E. coli O157:H7, among which 19 (10%) were resistant to sulfamethoxazole and 16 (8%) were resistant to tetracycline. The data suggest that selection pressure imposed by the use of tetracycline derivatives, sulfa drugs, cephalosporins, and penicillins, whether therapeutically in human and veterinary medicine or as prophylaxis in the animal production environment, is a key driving force in the selection of antimicrobial resistance in STEC and non-STEC O157.

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