Antimicrobial susceptibility testing using high surface-to-volume ratio microchannels

Chia Hsiang Chen, Yi Lu, Mandy L.Y. Sin, Kathleen E. Mach, Donna D. Zhang, Vincent Gau, Joseph C. Liao, Pak Kin Wong

Research output: Contribution to journalArticlepeer-review

88 Scopus citations

Abstract

This study reports the use of microfluidics, which intrinsically has a large surface-to-volume ratio, toward rapid antimicrobial susceptibility testing at the point of care. By observing the growth of uropathogenic Escherichia coli in gas permeable polymeric microchannels with different dimensions, we demonstrate that the large surface-tovolume ratio of microfluidic systems facilitates rapid growth of bacteria. For microchannels with 250 μm or less in depth, the effective oxygenation can sustain the growth of E. coli to over 109 cfu/mL without external agitation or oxygenation, which eliminates the requirement of bulky instrumentation and facilitates rapid bacterial growth for antimicrobial susceptibility testing at the point of care. The applicability of microfluidic rapid antimicrobial susceptibility testing is demonstrated in culture media and in urine with clinical bacterial isolates that have different antimicrobial resistance profiles. The antimicrobial resistance pattern can be determined as rapidly as 2 h compared to days in standard clinical procedures facilitating diagnostics at the point of care.

Original languageEnglish (US)
Pages (from-to)1012-1019
Number of pages8
JournalAnalytical chemistry
Volume82
Issue number3
DOIs
StatePublished - Feb 1 2010

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'Antimicrobial susceptibility testing using high surface-to-volume ratio microchannels'. Together they form a unique fingerprint.

Cite this