AOTF-based multicolor fluorescence detection for short tandem repeat (STR) analysis in an electrophoretic microdevice

James M. Karlinsey, James P. Landers

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

An acousto-optic tunable filter (AOTF) has been used to perform multicolor fluorescence detection for four and five-color short tandem repeat (STR) analysis on glass microchips. Matrix files were initially generated by collecting and comparing the laser-induced fluorescence emission of the labels specific to a particular STR kit, and raw data was processed to remove spectral overlap. The AmpFlSTR® kits used in this work include Profiler Plus™ and COfiler™, which are four-color kits used in tandem to address the core STR loci, as well as the five-color Identifiler™ kit, which contains each of the loci. In contrast to previous reports on multicolor detection for STR analysis on microchips, this detection system is characterized by a single filter and detector, and reports the first five-color genotyping application on-chip. This capability matches the portability and reduced scale of the microchip with the state-of-the-art in multicolor STR analysis kits.

Original languageEnglish (US)
Pages (from-to)1285-1291
Number of pages7
JournalLab on a Chip
Volume8
Issue number8
DOIs
StatePublished - Jan 1 2008

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Microsatellite Repeats
Optics
Fluorescence
Color
Microchip Analytical Procedures
Labels
Glass
Detectors
Lasers

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

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AOTF-based multicolor fluorescence detection for short tandem repeat (STR) analysis in an electrophoretic microdevice. / Karlinsey, James M.; Landers, James P.

In: Lab on a Chip, Vol. 8, No. 8, 01.01.2008, p. 1285-1291.

Research output: Contribution to journalArticle

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