Apparent Differential Response of Nuclear Envelope Cytochrome P-450 following Phenobarbital Induction Arising from a Preferential Loss during Gradient Purification

Gary A. Clawson, David E. Moody, C. H. Woo

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8 Scopus citations

Abstract

We have investigated the response of rat liver nuclear, nuclear envelope, and microsomal cytochrome P-450 (or P-448) to various treatments. Responses of these subcellular fractions to 3-methylcholanthrene pretreatment were generally similar. In endoplasmic reticulum preparations, we observed an increase in cytochrome P-450 content following phenobarbital pretreatment, which was reduced by subsequent thioacetamide treatment. Nuclear envelope cytochrome P-450 was apparently not modulated by these treatments, although nuclear cytochrome P-450 content was increased by phenobarbital. When endoplasmic reticulum preparations were subjected to treatments paralleling those used in nuclear envelope purification, we found a preferential loss of cytochrome P-450 from phenobarbital-pretreated preparations, with a loss of camphor-binding ability. The data point to potential problems with use of isolated nuclear envelopes as a representative model for nuclear metabolism of carcinogens, including low total recoveries and enrichments, and the potential for selective or differential recovery of cytochrome P-450 populations following various modes of induction or reduction. >.

Original languageEnglish (US)
Pages (from-to)3122-3127
Number of pages6
JournalCancer Research
Volume41
Issue number8
StatePublished - Aug 1 1981

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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