Apparent Differential Response of Nuclear Envelope Cytochrome P-450 following Phenobarbital Induction Arising from a Preferential Loss during Gradient Purification

Gary A. Clawson, David E. Moody, C. H. Woo

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We have investigated the response of rat liver nuclear, nuclear envelope, and microsomal cytochrome P-450 (or P-448) to various treatments. Responses of these subcellular fractions to 3-methylcholanthrene pretreatment were generally similar. In endoplasmic reticulum preparations, we observed an increase in cytochrome P-450 content following phenobarbital pretreatment, which was reduced by subsequent thioacetamide treatment. Nuclear envelope cytochrome P-450 was apparently not modulated by these treatments, although nuclear cytochrome P-450 content was increased by phenobarbital. When endoplasmic reticulum preparations were subjected to treatments paralleling those used in nuclear envelope purification, we found a preferential loss of cytochrome P-450 from phenobarbital-pretreated preparations, with a loss of camphor-binding ability. The data point to potential problems with use of isolated nuclear envelopes as a representative model for nuclear metabolism of carcinogens, including low total recoveries and enrichments, and the potential for selective or differential recovery of cytochrome P-450 populations following various modes of induction or reduction. >.

Original languageEnglish (US)
Pages (from-to)3122-3127
Number of pages6
JournalCancer Research
Volume41
Issue number8
StatePublished - Aug 1 1981

Fingerprint

Nuclear Envelope
Phenobarbital
Cytochrome P-450 Enzyme System
Endoplasmic Reticulum
Thioacetamide
Camphor
Methylcholanthrene
Subcellular Fractions
Carcinogens
Liver
Population

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

@article{5f82dcd458804bc1b17b28aa4edb1293,
title = "Apparent Differential Response of Nuclear Envelope Cytochrome P-450 following Phenobarbital Induction Arising from a Preferential Loss during Gradient Purification",
abstract = "We have investigated the response of rat liver nuclear, nuclear envelope, and microsomal cytochrome P-450 (or P-448) to various treatments. Responses of these subcellular fractions to 3-methylcholanthrene pretreatment were generally similar. In endoplasmic reticulum preparations, we observed an increase in cytochrome P-450 content following phenobarbital pretreatment, which was reduced by subsequent thioacetamide treatment. Nuclear envelope cytochrome P-450 was apparently not modulated by these treatments, although nuclear cytochrome P-450 content was increased by phenobarbital. When endoplasmic reticulum preparations were subjected to treatments paralleling those used in nuclear envelope purification, we found a preferential loss of cytochrome P-450 from phenobarbital-pretreated preparations, with a loss of camphor-binding ability. The data point to potential problems with use of isolated nuclear envelopes as a representative model for nuclear metabolism of carcinogens, including low total recoveries and enrichments, and the potential for selective or differential recovery of cytochrome P-450 populations following various modes of induction or reduction. >.",
author = "Clawson, {Gary A.} and Moody, {David E.} and Woo, {C. H.}",
year = "1981",
month = "8",
day = "1",
language = "English (US)",
volume = "41",
pages = "3122--3127",
journal = "Cancer Research",
issn = "0008-5472",
number = "8",

}

Apparent Differential Response of Nuclear Envelope Cytochrome P-450 following Phenobarbital Induction Arising from a Preferential Loss during Gradient Purification. / Clawson, Gary A.; Moody, David E.; Woo, C. H.

In: Cancer Research, Vol. 41, No. 8, 01.08.1981, p. 3122-3127.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Apparent Differential Response of Nuclear Envelope Cytochrome P-450 following Phenobarbital Induction Arising from a Preferential Loss during Gradient Purification

AU - Clawson, Gary A.

AU - Moody, David E.

AU - Woo, C. H.

PY - 1981/8/1

Y1 - 1981/8/1

N2 - We have investigated the response of rat liver nuclear, nuclear envelope, and microsomal cytochrome P-450 (or P-448) to various treatments. Responses of these subcellular fractions to 3-methylcholanthrene pretreatment were generally similar. In endoplasmic reticulum preparations, we observed an increase in cytochrome P-450 content following phenobarbital pretreatment, which was reduced by subsequent thioacetamide treatment. Nuclear envelope cytochrome P-450 was apparently not modulated by these treatments, although nuclear cytochrome P-450 content was increased by phenobarbital. When endoplasmic reticulum preparations were subjected to treatments paralleling those used in nuclear envelope purification, we found a preferential loss of cytochrome P-450 from phenobarbital-pretreated preparations, with a loss of camphor-binding ability. The data point to potential problems with use of isolated nuclear envelopes as a representative model for nuclear metabolism of carcinogens, including low total recoveries and enrichments, and the potential for selective or differential recovery of cytochrome P-450 populations following various modes of induction or reduction. >.

AB - We have investigated the response of rat liver nuclear, nuclear envelope, and microsomal cytochrome P-450 (or P-448) to various treatments. Responses of these subcellular fractions to 3-methylcholanthrene pretreatment were generally similar. In endoplasmic reticulum preparations, we observed an increase in cytochrome P-450 content following phenobarbital pretreatment, which was reduced by subsequent thioacetamide treatment. Nuclear envelope cytochrome P-450 was apparently not modulated by these treatments, although nuclear cytochrome P-450 content was increased by phenobarbital. When endoplasmic reticulum preparations were subjected to treatments paralleling those used in nuclear envelope purification, we found a preferential loss of cytochrome P-450 from phenobarbital-pretreated preparations, with a loss of camphor-binding ability. The data point to potential problems with use of isolated nuclear envelopes as a representative model for nuclear metabolism of carcinogens, including low total recoveries and enrichments, and the potential for selective or differential recovery of cytochrome P-450 populations following various modes of induction or reduction. >.

UR - http://www.scopus.com/inward/record.url?scp=0019458972&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019458972&partnerID=8YFLogxK

M3 - Article

C2 - 6788368

AN - SCOPUS:0019458972

VL - 41

SP - 3122

EP - 3127

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 8

ER -