Application of automated image analysis to demonstrate the correlation between ras p21 expression and severity of gliomas

Michael Salgaller, Lawrence Agius, Allan Yates, Dennis Keith Pearl, William Roberts, Ralph Stephens

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

We found a direct correlation between increasing ras p21 protein immunopositivity and severity of human glioma using computer-assisted, digital-image processing to quantify the amount of p21 immunoreactive to the monoclonal antibody RAP-5. We determined that there was a significant difference in reactivity between glioblastoma multiformes and more-differentiated astrocytomas (experiment-wise error <0.05). This result confirmed the conclusions made on the same tumors using standard light microscopy and visual examination. Immunohistochemistry quantized by automated image analysis may be a useful adjunct to current histopathological strategies since it decreases assay subjectivity and variation.

Original languageEnglish (US)
Pages (from-to)482-491
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume169
Issue number2
DOIs
StatePublished - Jun 15 1990

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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