We found a direct correlation between increasing ras p21 protein immunopositivity and severity of human glioma using computer-assisted, digital-image processing to quantify the amount of p21 immunoreactive to the monoclonal antibody RAP-5. We determined that there was a significant difference in reactivity between glioblastoma multiformes and more-differentiated astrocytomas (experiment-wise error <0.05). This result confirmed the conclusions made on the same tumors using standard light microscopy and visual examination. Immunohistochemistry quantized by automated image analysis may be a useful adjunct to current histopathological strategies since it decreases assay subjectivity and variation.
|Original language||English (US)|
|Number of pages||10|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jun 15 1990|
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology