Application of carnatrol® and timsen® to decontaminate beef

Catherine Nettles Cutter, Warren J. Dorsa, Gregory R. Siragusa

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The spray application of two commercial decontaminating agents for reducing bacterial populations associated with fecal contamination on beef was examined in two separate experiments. Individual pieces of prerigor lean beef tissue were inoculated with fresh bovine feces and subjected to a 15-s spray wash (75 Ib/in2, 20°C) with water or various concentrations of Carnatrol®, composed of copper sulfate pentahydrate, or Timsen®, 40% N-alkyldimethylbenzylammonium chloride in 60% stabilized urea, and stored under refrigerated (5°C) conditions, When Carnatrol® was applied to beef tissue at 20, 40, and 80 ppm, bacterial populations were not statistically different (P ≥ 0.05) than water-treated populations at days 0, 1, and 2. When Carnatrol® was applied to tissues at 160 ppm, bacterial populations were statistically different (P ≤ 0.05) from water-treated tissue on all of the days examined; however, reductions were not greater than 0.58, 0.42, and 0.35 log CFU/cm2 at days 0, 1, and 2, respectively. Remaining bacterial populations resulting from spray applications of Timsen® to tissues at 200, 400, and 800 ppm were not statistically different than remaining bacterial populations of water-treated tissues at days 0, 1, 2, or 3. Reductions in bacterial populations associated with Timsen® were no greater than 0.40 log CFU/cm2 on any of the days examined. This study demonstrates that under conditions used in this study, spray washes with either of the two commercially available decontaminating agents were no more effective than water washes for reducing bacterial populations associated with fecal contamination on beef tissue.

Original languageEnglish (US)
Pages (from-to)1339-1342
Number of pages4
JournalJournal of Food Protection
Volume59
Issue number12
DOIs
StatePublished - Jan 1 1996

Fingerprint

beef
Population
Water
water
Benzalkonium Compounds
Copper Sulfate
reducing agents
copper sulfate
Red Meat
Reducing Agents
tissues
Feces
Urea
chlorides
urea
feces
cattle

All Science Journal Classification (ASJC) codes

  • Food Science
  • Microbiology

Cite this

Cutter, Catherine Nettles ; Dorsa, Warren J. ; Siragusa, Gregory R. / Application of carnatrol® and timsen® to decontaminate beef. In: Journal of Food Protection. 1996 ; Vol. 59, No. 12. pp. 1339-1342.
@article{f120fe09d3714320ade35ab6c43e382e,
title = "Application of carnatrol{\circledR} and timsen{\circledR} to decontaminate beef",
abstract = "The spray application of two commercial decontaminating agents for reducing bacterial populations associated with fecal contamination on beef was examined in two separate experiments. Individual pieces of prerigor lean beef tissue were inoculated with fresh bovine feces and subjected to a 15-s spray wash (75 Ib/in2, 20°C) with water or various concentrations of Carnatrol{\circledR}, composed of copper sulfate pentahydrate, or Timsen{\circledR}, 40{\%} N-alkyldimethylbenzylammonium chloride in 60{\%} stabilized urea, and stored under refrigerated (5°C) conditions, When Carnatrol{\circledR} was applied to beef tissue at 20, 40, and 80 ppm, bacterial populations were not statistically different (P ≥ 0.05) than water-treated populations at days 0, 1, and 2. When Carnatrol{\circledR} was applied to tissues at 160 ppm, bacterial populations were statistically different (P ≤ 0.05) from water-treated tissue on all of the days examined; however, reductions were not greater than 0.58, 0.42, and 0.35 log CFU/cm2 at days 0, 1, and 2, respectively. Remaining bacterial populations resulting from spray applications of Timsen{\circledR} to tissues at 200, 400, and 800 ppm were not statistically different than remaining bacterial populations of water-treated tissues at days 0, 1, 2, or 3. Reductions in bacterial populations associated with Timsen{\circledR} were no greater than 0.40 log CFU/cm2 on any of the days examined. This study demonstrates that under conditions used in this study, spray washes with either of the two commercially available decontaminating agents were no more effective than water washes for reducing bacterial populations associated with fecal contamination on beef tissue.",
author = "Cutter, {Catherine Nettles} and Dorsa, {Warren J.} and Siragusa, {Gregory R.}",
year = "1996",
month = "1",
day = "1",
doi = "10.4315/0362-028X-59.12.1339",
language = "English (US)",
volume = "59",
pages = "1339--1342",
journal = "Journal of Food Protection",
issn = "0362-028X",
publisher = "International Association for Food Protection",
number = "12",

}

Application of carnatrol® and timsen® to decontaminate beef. / Cutter, Catherine Nettles; Dorsa, Warren J.; Siragusa, Gregory R.

In: Journal of Food Protection, Vol. 59, No. 12, 01.01.1996, p. 1339-1342.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Application of carnatrol® and timsen® to decontaminate beef

AU - Cutter, Catherine Nettles

AU - Dorsa, Warren J.

AU - Siragusa, Gregory R.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - The spray application of two commercial decontaminating agents for reducing bacterial populations associated with fecal contamination on beef was examined in two separate experiments. Individual pieces of prerigor lean beef tissue were inoculated with fresh bovine feces and subjected to a 15-s spray wash (75 Ib/in2, 20°C) with water or various concentrations of Carnatrol®, composed of copper sulfate pentahydrate, or Timsen®, 40% N-alkyldimethylbenzylammonium chloride in 60% stabilized urea, and stored under refrigerated (5°C) conditions, When Carnatrol® was applied to beef tissue at 20, 40, and 80 ppm, bacterial populations were not statistically different (P ≥ 0.05) than water-treated populations at days 0, 1, and 2. When Carnatrol® was applied to tissues at 160 ppm, bacterial populations were statistically different (P ≤ 0.05) from water-treated tissue on all of the days examined; however, reductions were not greater than 0.58, 0.42, and 0.35 log CFU/cm2 at days 0, 1, and 2, respectively. Remaining bacterial populations resulting from spray applications of Timsen® to tissues at 200, 400, and 800 ppm were not statistically different than remaining bacterial populations of water-treated tissues at days 0, 1, 2, or 3. Reductions in bacterial populations associated with Timsen® were no greater than 0.40 log CFU/cm2 on any of the days examined. This study demonstrates that under conditions used in this study, spray washes with either of the two commercially available decontaminating agents were no more effective than water washes for reducing bacterial populations associated with fecal contamination on beef tissue.

AB - The spray application of two commercial decontaminating agents for reducing bacterial populations associated with fecal contamination on beef was examined in two separate experiments. Individual pieces of prerigor lean beef tissue were inoculated with fresh bovine feces and subjected to a 15-s spray wash (75 Ib/in2, 20°C) with water or various concentrations of Carnatrol®, composed of copper sulfate pentahydrate, or Timsen®, 40% N-alkyldimethylbenzylammonium chloride in 60% stabilized urea, and stored under refrigerated (5°C) conditions, When Carnatrol® was applied to beef tissue at 20, 40, and 80 ppm, bacterial populations were not statistically different (P ≥ 0.05) than water-treated populations at days 0, 1, and 2. When Carnatrol® was applied to tissues at 160 ppm, bacterial populations were statistically different (P ≤ 0.05) from water-treated tissue on all of the days examined; however, reductions were not greater than 0.58, 0.42, and 0.35 log CFU/cm2 at days 0, 1, and 2, respectively. Remaining bacterial populations resulting from spray applications of Timsen® to tissues at 200, 400, and 800 ppm were not statistically different than remaining bacterial populations of water-treated tissues at days 0, 1, 2, or 3. Reductions in bacterial populations associated with Timsen® were no greater than 0.40 log CFU/cm2 on any of the days examined. This study demonstrates that under conditions used in this study, spray washes with either of the two commercially available decontaminating agents were no more effective than water washes for reducing bacterial populations associated with fecal contamination on beef tissue.

UR - http://www.scopus.com/inward/record.url?scp=0030445979&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030445979&partnerID=8YFLogxK

U2 - 10.4315/0362-028X-59.12.1339

DO - 10.4315/0362-028X-59.12.1339

M3 - Article

VL - 59

SP - 1339

EP - 1342

JO - Journal of Food Protection

JF - Journal of Food Protection

SN - 0362-028X

IS - 12

ER -