Application of population dynamics to study heterotypic cell aggregations in the near-wall region of a shear flow

Yanping Ma, Jiakou Wang, Shile Liang, Cheng Dong, Qiang Du

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Our research focused on the polymorphonuclear neutrophils (PMNs) tethering to the vascular endothelial cells (EC) and the subsequent melanoma cell emboli formation in a shear flow, an important process of tumor cell extravasation from the circulation during metastasis. We applied population balance model based on Smoluchowski coagulation equation to study the heterotypic aggregation between PMNs and melanoma cells in the near-wall region of an in vitro parallel-plate flow chamber, which simulates in vivo cell-substrate adhesion from the vasculatures by combining mathematical modeling and numerical simulations with experimental observations. To the best of our knowledge, a multiscale near-wall aggregation model was developed, for the first time, which incorporated the effects of both cell deformation and general ratios of heterotypic cells on the cell aggregation process. Quantitative agreement was found between numerical predictions and in vitro experiments. The effects of factors, including: Intrinsic binding molecule properties, near-wall heterotypic cell concentrations, and cell deformations on the coagulation process, are discussed. Several parameter identification approaches are proposed and validated which, in turn, demonstrate the importance of the reaction coefficient and the critical bond number on the aggregation process.

Original languageEnglish (US)
Pages (from-to)3-19
Number of pages17
JournalCellular and Molecular Bioengineering
Volume3
Issue number1
DOIs
StatePublished - Mar 1 2010

Fingerprint

Cell Aggregation
Population dynamics
Population Dynamics
Shear flow
Shear Flow
Aggregation
Agglomeration
Cell
Coagulation
Neutrophils
Melanoma
Endothelial cells
Tumors
Identification (control systems)
Intrinsic Factor
Adhesion
Population Balance
Cell Formation
Cells
Endothelial Cells

All Science Journal Classification (ASJC) codes

  • Modeling and Simulation
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

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abstract = "Our research focused on the polymorphonuclear neutrophils (PMNs) tethering to the vascular endothelial cells (EC) and the subsequent melanoma cell emboli formation in a shear flow, an important process of tumor cell extravasation from the circulation during metastasis. We applied population balance model based on Smoluchowski coagulation equation to study the heterotypic aggregation between PMNs and melanoma cells in the near-wall region of an in vitro parallel-plate flow chamber, which simulates in vivo cell-substrate adhesion from the vasculatures by combining mathematical modeling and numerical simulations with experimental observations. To the best of our knowledge, a multiscale near-wall aggregation model was developed, for the first time, which incorporated the effects of both cell deformation and general ratios of heterotypic cells on the cell aggregation process. Quantitative agreement was found between numerical predictions and in vitro experiments. The effects of factors, including: Intrinsic binding molecule properties, near-wall heterotypic cell concentrations, and cell deformations on the coagulation process, are discussed. Several parameter identification approaches are proposed and validated which, in turn, demonstrate the importance of the reaction coefficient and the critical bond number on the aggregation process.",
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Application of population dynamics to study heterotypic cell aggregations in the near-wall region of a shear flow. / Ma, Yanping; Wang, Jiakou; Liang, Shile; Dong, Cheng; Du, Qiang.

In: Cellular and Molecular Bioengineering, Vol. 3, No. 1, 01.03.2010, p. 3-19.

Research output: Contribution to journalArticle

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