Application of RAPDs to DNA extracted from apple rootstocks

Wesley R. Autio, James Rawlinson Schupp, David C. Ferree, Richard Glavin, David L. Mulcahy

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Samples of 15 different rootstocks from mature apple trees (Malus xdomestica Borkh.) with 'Starkspur Supreme Delicious' as the scion were differentiated adequately using random amplified polymorphic DNAs (RAPDs). A procedure for extraction of DNA from root material is described. Patterns of DNA from leaf tissue of young trees of 10 of the rootstocks were compared with those from root tissue of mature trees grafted on the same rootstocks and at a different location to reaffirm identification of these rootstocks. Similar patterns were obtained for a) root vs. leaf tissue and b) tissue from two locations. Except for one apparent misidentification (within a single replication), patterns for root tissue of all rootstocks matched those of the corresponding leaf tissue.

Original languageEnglish (US)
Pages (from-to)333-335
Number of pages3
JournalHortScience
Volume33
Issue number2
StatePublished - Jan 1 1998

All Science Journal Classification (ASJC) codes

  • Horticulture

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