Application of recombinant fimbrial protein for the specific detection of Salmonella enteritidis infection in poultry

Gireesh Rajashekara, Shirin Munir, Chinta M. Lamichhane, Alberto Back, Vivek Kapur, David A. Halvorson, Kakambi V. Nagaraja

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

A number of disease outbreaks of Salmonella enterica serotype enteritidis (SE) in humans have been traced to the consumption of SE-contaminated egg and egg products. A rapid, specific, and inexpensive method of detecting SE infection in poultry is necessary to reduce human outbreaks. We evaluated rSEF14 fimbrial antigen of SE for specific detection of SE-infected birds in latex agglutination test and enzyme-linked immunosorbent assay. rSEF14 antigen was highly specific in identifying birds infected with SE. The sera from birds infected with closely related serogroup-D Salmonella and other avian pathogens did not react with rSEF14 antigen. The rSEF14 antigen identified antibodies in serum of 88% of birds during the first 2 weeks of infection, and 100% of the birds subsequently. The SE-specific antibodies were detected in egg yolk as early as 6 days post-infection in rSEF14-enzyme-linked immunosorbent assay. Our results suggest that rSEF14-based assays could be used as screening tests for detection of SE antibodies and would overcome the cross reactions observed with existing serological tests. Copyright (C) 1998 Elsevier Science Inc.

Original languageEnglish (US)
Pages (from-to)147-157
Number of pages11
JournalDiagnostic Microbiology and Infectious Disease
Volume32
Issue number3
DOIs
StatePublished - Nov 1 1998

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

Cite this