Approaches for hybrid biological and synthetic system integration

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Eukaryotic cells use cytoskeletal tracks known as microtubules and motor proteins such as kinesin for transporting intracellular cargo and for positioning organelles. Kinesin motors move unidirectionally along microtubules using the energy from hydrolysis of adenosine triphosphate (ATP). Studies have shown that the forces generated by the kinesin motors are sufficient to mobilize synthetic devices. A critical component in realizing useful work from the directional motion of biomolecular motor protein systems involves patterning kinesin motors in pre-defined areas and aligning microtubules on various surfaces. In order to reduce another degree of freedom in such systems both kinesin and microtubules need to be patterned simultaneously. Traditional device fabrication processes such as lithography, however, are not considered compatible with the biological proteins. Accordingly in previous studies devices were fabricated first and functional proteins immobilized on them subsequently. To create hybrid biological and synthetic systems it may be necessary to integrate biological entities such as proteins with device fabrication processes. Toward this end, a series of experiments were conducted to investigate the compatibility of neutravidin and biotinylated kinesin with acetone, remover of electron beam resist poly methyl methacrylate (PMMA). It is found that neutravidin and biotinylated kinesin retain their functionality after acetone exposure. It is also found that exposure to PMMA does not defunctionalize. These studies open up a new approach for the micro and nanoscale patterning of biological molecular motors.

Original languageEnglish (US)
Title of host publicationProceedings - 2005 International Symposium on Microelectronics, IMAPS 2005
Pages244-249
Number of pages6
StatePublished - Dec 1 2005
Event38th International Symposium on Microelectronics, IMAPS 2005 - Philadelphia, PA, United States
Duration: Sep 25 2005Sep 29 2005

Publication series

NameProceedings - 2005 International Symposium on Microelectronics, IMAPS 2005

Other

Other38th International Symposium on Microelectronics, IMAPS 2005
CountryUnited States
CityPhiladelphia, PA
Period9/25/059/29/05

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

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    Verma, V., Hancock, W. O., & Catchmark, J. M. (2005). Approaches for hybrid biological and synthetic system integration. In Proceedings - 2005 International Symposium on Microelectronics, IMAPS 2005 (pp. 244-249). (Proceedings - 2005 International Symposium on Microelectronics, IMAPS 2005).