Approaches to the development of assays for interaction of tobacco-specific nitrosamines with haemoglobin and DNA.

S. S. Hecht, S. G. Carmella, N. Trushin, Thomas Spratt, P. G. Foiles, D. Hoffmann

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Abstract

The tobacco-specific, nicotine-derived nitrosamines 4-(N-nitrosomethylamino)-1-(3-pyridyl)-1-butanone (NNK) and N'-nitrosonornicotine (NNN) are among the most important carcinogens in tobacco and tobacco smoke. Treatment of Fischer 344 rats with these carcinogens resulted in alkylation of haemoglobin and DNA by the 4-(3-pyridyl)-4-oxobutyl group formed during their metabolism. This alkyl group can be detached from globin or DNA under mild hydrolytic conditions as 4-hydroxy-1-(3-pyridyl)-1-butanone, which appears to be a potentially useful dosimeter for human exposure to, and activation of, tobacco-specific nitrosamines.

Original languageEnglish (US)
Pages (from-to)121-128
Number of pages8
JournalIARC scientific publications
Issue number89
Publication statusPublished - Dec 1 1988

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All Science Journal Classification (ASJC) codes

  • Medicine(all)

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