Arabidopsis cell division cycle 20.1 is required for normal meiotic spindle assembly and chromosome segregation

Baixiao Niu, Liudan Wang, Liangsheng Zhang, Ding Ren, Ren Ren, Gregory P. Copenhaver, Hong Ma, Yingxiang Wang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Cell division requires proper spindle assembly; a surveillance pathway, the spindle assembly checkpoint (SAC), monitors whether the spindle is normal and correctly attached to kinetochores. The SAC proteins regulate mitotic chromosome segregation by affecting CDC20 (Cell Division Cycle 20) function. However, it is unclear whether CDC20 regulates meiotic spindle assembly and proper homolog segregation. Here, we show that the Arabidopsis thaliana CDC20.1 gene is indispensable for meiosis and male fertility. We demonstrate that cdc20.1 meiotic chromosomes align asynchronously and segregate unequally and the metaphase I spindle has aberrant morphology. Comparison of the distribution of meiotic stages at different time points between the wild type and cdc20.1 reveals a delay of meiotic progression from diakinesis to anaphase I. Furthermore, cdc20.1 meiocytes exhibit an abnormal distribution of a histone H3 phosphorylation mark mediated by the Aurora kinase, providing evidence that CDC20.1 regulates Aurora localization for meiotic chromosome segregation. Further evidence that CDC20.1 and Aurora are functionally related was provided by meiosis-specific knockdown of At-Aurora1 expression, resulting in meiotic chromosome segregation defects similar to those of cdc20.1. Taken together, these results suggest a critical role for CDC20.1 in SAC-dependent meiotic chromosome segregation.

Original languageEnglish (US)
Pages (from-to)3367-3382
Number of pages16
JournalPlant Cell
Volume27
Issue number12
DOIs
StatePublished - Dec 1 2015

Fingerprint

Chromosome Segregation
Spindle Apparatus
chromosome segregation
M Phase Cell Cycle Checkpoints
Arabidopsis
cell division
Cell Cycle
Meiosis
meiosis
Aurora Kinases
Meiotic Prophase I
Kinetochores
kinetochores
Anaphase
monitoring
male fertility
anaphase
prophase
Metaphase
metaphase

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

Niu, B., Wang, L., Zhang, L., Ren, D., Ren, R., Copenhaver, G. P., ... Wang, Y. (2015). Arabidopsis cell division cycle 20.1 is required for normal meiotic spindle assembly and chromosome segregation. Plant Cell, 27(12), 3367-3382. https://doi.org/10.1105/tpc.15.00834
Niu, Baixiao ; Wang, Liudan ; Zhang, Liangsheng ; Ren, Ding ; Ren, Ren ; Copenhaver, Gregory P. ; Ma, Hong ; Wang, Yingxiang. / Arabidopsis cell division cycle 20.1 is required for normal meiotic spindle assembly and chromosome segregation. In: Plant Cell. 2015 ; Vol. 27, No. 12. pp. 3367-3382.
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abstract = "Cell division requires proper spindle assembly; a surveillance pathway, the spindle assembly checkpoint (SAC), monitors whether the spindle is normal and correctly attached to kinetochores. The SAC proteins regulate mitotic chromosome segregation by affecting CDC20 (Cell Division Cycle 20) function. However, it is unclear whether CDC20 regulates meiotic spindle assembly and proper homolog segregation. Here, we show that the Arabidopsis thaliana CDC20.1 gene is indispensable for meiosis and male fertility. We demonstrate that cdc20.1 meiotic chromosomes align asynchronously and segregate unequally and the metaphase I spindle has aberrant morphology. Comparison of the distribution of meiotic stages at different time points between the wild type and cdc20.1 reveals a delay of meiotic progression from diakinesis to anaphase I. Furthermore, cdc20.1 meiocytes exhibit an abnormal distribution of a histone H3 phosphorylation mark mediated by the Aurora kinase, providing evidence that CDC20.1 regulates Aurora localization for meiotic chromosome segregation. Further evidence that CDC20.1 and Aurora are functionally related was provided by meiosis-specific knockdown of At-Aurora1 expression, resulting in meiotic chromosome segregation defects similar to those of cdc20.1. Taken together, these results suggest a critical role for CDC20.1 in SAC-dependent meiotic chromosome segregation.",
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Niu, B, Wang, L, Zhang, L, Ren, D, Ren, R, Copenhaver, GP, Ma, H & Wang, Y 2015, 'Arabidopsis cell division cycle 20.1 is required for normal meiotic spindle assembly and chromosome segregation', Plant Cell, vol. 27, no. 12, pp. 3367-3382. https://doi.org/10.1105/tpc.15.00834

Arabidopsis cell division cycle 20.1 is required for normal meiotic spindle assembly and chromosome segregation. / Niu, Baixiao; Wang, Liudan; Zhang, Liangsheng; Ren, Ding; Ren, Ren; Copenhaver, Gregory P.; Ma, Hong; Wang, Yingxiang.

In: Plant Cell, Vol. 27, No. 12, 01.12.2015, p. 3367-3382.

Research output: Contribution to journalArticle

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AU - Niu, Baixiao

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AU - Copenhaver, Gregory P.

AU - Ma, Hong

AU - Wang, Yingxiang

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