Arabidopsis glutaredoxin S17 contributes to vegetative growth, mineral accumulation, and redox balance during iron deficiency

Han Yu, Jian Yang, Yafei Shi, Jimmonique Donelson, Sean M. Thompson, Stuart Sprague, Tony Roshan, Da Li Wang, Jianzhong Liu, Sunghun Park, Paul A. Nakata, Erin L. Connolly, Kendal D. Hirschi, Michael A. Grusak, Ninghui Cheng

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3 Citations (Scopus)

Abstract

Iron (Fe) is an essential mineral nutrient and a metal cofactor required for many proteins and enzymes involved in the processes of DNA synthesis, respiration, and photosynthesis. Iron limitation can have detrimental effects on plant growth and development. Such effects are mediated, at least in part, through the generation of reactive oxygen species (ROS). Thus, plants have evolved a complex regulatory network to respond to conditions of iron limitations. However, the mechanisms that couple iron deficiency and oxidative stress responses are not fully understood. Here, we report the discovery that an Arabidopsis thaliana monothiol glutaredoxin S17 (AtGRXS17) plays a critical role in the plants ability to respond to iron deficiency stress and maintain redox homeostasis. In a yeast expression assay, AtGRXS17 was able to suppress the iron accumulation in yeast ScGrx3/ScGrx4 mutant cells. Genetic analysis indicated that plants with reduced AtGRXS17 expression were hypersensitive to iron deficiency and showed increased iron concentrations in mature seeds. Disruption of AtGRXS17 caused plant sensitivity to exogenous oxidants and increased ROS production under iron deficiency. Addition of reduced glutathione rescued the growth and alleviates the sensitivity of atgrxs17 mutants to iron deficiency. These findings suggest AtGRXS17 helps integrate redox homeostasis and iron deficiency responses.

Original languageEnglish (US)
Article number1045
JournalFrontiers in Plant Science
Volume8
DOIs
StatePublished - Jun 19 2017

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vegetative growth
Arabidopsis
iron
minerals
Arabidopsis thaliana
reactive oxygen species
homeostasis
yeasts
mutants
cell respiration
oxidants
genetic techniques and protocols
plant development
glutathione
stress response
growth and development
oxidative stress
metals
plant growth
photosynthesis

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

Yu, Han ; Yang, Jian ; Shi, Yafei ; Donelson, Jimmonique ; Thompson, Sean M. ; Sprague, Stuart ; Roshan, Tony ; Wang, Da Li ; Liu, Jianzhong ; Park, Sunghun ; Nakata, Paul A. ; Connolly, Erin L. ; Hirschi, Kendal D. ; Grusak, Michael A. ; Cheng, Ninghui. / Arabidopsis glutaredoxin S17 contributes to vegetative growth, mineral accumulation, and redox balance during iron deficiency. In: Frontiers in Plant Science. 2017 ; Vol. 8.
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abstract = "Iron (Fe) is an essential mineral nutrient and a metal cofactor required for many proteins and enzymes involved in the processes of DNA synthesis, respiration, and photosynthesis. Iron limitation can have detrimental effects on plant growth and development. Such effects are mediated, at least in part, through the generation of reactive oxygen species (ROS). Thus, plants have evolved a complex regulatory network to respond to conditions of iron limitations. However, the mechanisms that couple iron deficiency and oxidative stress responses are not fully understood. Here, we report the discovery that an Arabidopsis thaliana monothiol glutaredoxin S17 (AtGRXS17) plays a critical role in the plants ability to respond to iron deficiency stress and maintain redox homeostasis. In a yeast expression assay, AtGRXS17 was able to suppress the iron accumulation in yeast ScGrx3/ScGrx4 mutant cells. Genetic analysis indicated that plants with reduced AtGRXS17 expression were hypersensitive to iron deficiency and showed increased iron concentrations in mature seeds. Disruption of AtGRXS17 caused plant sensitivity to exogenous oxidants and increased ROS production under iron deficiency. Addition of reduced glutathione rescued the growth and alleviates the sensitivity of atgrxs17 mutants to iron deficiency. These findings suggest AtGRXS17 helps integrate redox homeostasis and iron deficiency responses.",
author = "Han Yu and Jian Yang and Yafei Shi and Jimmonique Donelson and Thompson, {Sean M.} and Stuart Sprague and Tony Roshan and Wang, {Da Li} and Jianzhong Liu and Sunghun Park and Nakata, {Paul A.} and Connolly, {Erin L.} and Hirschi, {Kendal D.} and Grusak, {Michael A.} and Ninghui Cheng",
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Yu, H, Yang, J, Shi, Y, Donelson, J, Thompson, SM, Sprague, S, Roshan, T, Wang, DL, Liu, J, Park, S, Nakata, PA, Connolly, EL, Hirschi, KD, Grusak, MA & Cheng, N 2017, 'Arabidopsis glutaredoxin S17 contributes to vegetative growth, mineral accumulation, and redox balance during iron deficiency', Frontiers in Plant Science, vol. 8, 1045. https://doi.org/10.3389/fpls.2017.01045

Arabidopsis glutaredoxin S17 contributes to vegetative growth, mineral accumulation, and redox balance during iron deficiency. / Yu, Han; Yang, Jian; Shi, Yafei; Donelson, Jimmonique; Thompson, Sean M.; Sprague, Stuart; Roshan, Tony; Wang, Da Li; Liu, Jianzhong; Park, Sunghun; Nakata, Paul A.; Connolly, Erin L.; Hirschi, Kendal D.; Grusak, Michael A.; Cheng, Ninghui.

In: Frontiers in Plant Science, Vol. 8, 1045, 19.06.2017.

Research output: Contribution to journalArticle

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T1 - Arabidopsis glutaredoxin S17 contributes to vegetative growth, mineral accumulation, and redox balance during iron deficiency

AU - Yu, Han

AU - Yang, Jian

AU - Shi, Yafei

AU - Donelson, Jimmonique

AU - Thompson, Sean M.

AU - Sprague, Stuart

AU - Roshan, Tony

AU - Wang, Da Li

AU - Liu, Jianzhong

AU - Park, Sunghun

AU - Nakata, Paul A.

AU - Connolly, Erin L.

AU - Hirschi, Kendal D.

AU - Grusak, Michael A.

AU - Cheng, Ninghui

PY - 2017/6/19

Y1 - 2017/6/19

N2 - Iron (Fe) is an essential mineral nutrient and a metal cofactor required for many proteins and enzymes involved in the processes of DNA synthesis, respiration, and photosynthesis. Iron limitation can have detrimental effects on plant growth and development. Such effects are mediated, at least in part, through the generation of reactive oxygen species (ROS). Thus, plants have evolved a complex regulatory network to respond to conditions of iron limitations. However, the mechanisms that couple iron deficiency and oxidative stress responses are not fully understood. Here, we report the discovery that an Arabidopsis thaliana monothiol glutaredoxin S17 (AtGRXS17) plays a critical role in the plants ability to respond to iron deficiency stress and maintain redox homeostasis. In a yeast expression assay, AtGRXS17 was able to suppress the iron accumulation in yeast ScGrx3/ScGrx4 mutant cells. Genetic analysis indicated that plants with reduced AtGRXS17 expression were hypersensitive to iron deficiency and showed increased iron concentrations in mature seeds. Disruption of AtGRXS17 caused plant sensitivity to exogenous oxidants and increased ROS production under iron deficiency. Addition of reduced glutathione rescued the growth and alleviates the sensitivity of atgrxs17 mutants to iron deficiency. These findings suggest AtGRXS17 helps integrate redox homeostasis and iron deficiency responses.

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