Arabidopsis MALE STERILITY1 encodes a PHD-type transcription factor and regulates pollen and tapetum development

Takuya Ito, Noriko Nagata, Yoshu Yoshiba, Masaru Ohme-Takagi, Hong Ma, Kazuo Shinozakif

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

The Arabidopsis thaliana MALE STERILITY1 (MS1)gene encodes a nuclear protein with Leu zipper-like and PHD-finger motifs and is important for postmeiotic pollen development. Here, we examined MS1 function using both cell biological and molecular biological approaches. We introduced a fusion construct of MS1 and a transcriptional repression domain (MS1-SRDX) into wildtype Arabidopsis, and the transgenic plants showed a semisterile phenotype similar to that of ms1. Since the repression domain can convert various kinds of transcriptional activators to dominant repressors, this suggested that MS1 functioned as a transcriptional activator. The Leu zipper-like region and the PHD motif were required for the MS1 function. Phenotypic analysis of the ms1 mutant and the MS1-SRDX transgenic Arabidopsis indicated that MS1 was involved information of pollen exine and pollen cytosolic components as well as tapetum development. Next, we searched for MS1 downstream genes by analyzing publicly available microarray data and identified 95 genes affected by MS1. Using a transgenic ms1 plant showing dexamethasone-inducible recovery of fertility, we further examined whether these genes were immediately downstream of MS1. From these results, we discuss a role of MS1 in pollen and tapetum development and the conservation of MS1 function in flowering plants.

Original languageEnglish (US)
Pages (from-to)3549-3562
Number of pages14
JournalPlant Cell
Volume19
Issue number11
DOIs
StatePublished - Nov 1 2007

Fingerprint

Pollen
Arabidopsis
Transcription Factors
transcription factors
pollen
Genetically Modified Plants
Genes
transgenic plants
genes
exine
nuclear proteins
dexamethasone
Nuclear Proteins
Dexamethasone
Fingers
Fertility
Angiospermae
Arabidopsis thaliana
genetically modified organisms
Phenotype

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

Ito, Takuya ; Nagata, Noriko ; Yoshiba, Yoshu ; Ohme-Takagi, Masaru ; Ma, Hong ; Shinozakif, Kazuo. / Arabidopsis MALE STERILITY1 encodes a PHD-type transcription factor and regulates pollen and tapetum development. In: Plant Cell. 2007 ; Vol. 19, No. 11. pp. 3549-3562.
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Arabidopsis MALE STERILITY1 encodes a PHD-type transcription factor and regulates pollen and tapetum development. / Ito, Takuya; Nagata, Noriko; Yoshiba, Yoshu; Ohme-Takagi, Masaru; Ma, Hong; Shinozakif, Kazuo.

In: Plant Cell, Vol. 19, No. 11, 01.11.2007, p. 3549-3562.

Research output: Contribution to journalArticle

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