Arabidopsis PARG1 is the key factor promoting cell survival among the enzymes regulating post-translational poly(ADP-ribosyl)ation

Hailei Zhang, Zongying Gu, Qiao Wu, Lifeng Yang, Caifeng Liu, Hong Ma, Yiji Xia, Xiaochun Ge

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Poly(ADP-ribosyl)ation is a reversible post-translational modification of proteins, characterized by the addition of poly(ADP-ribose) (PAR) to proteins by poly(ADP-ribose) polymerase (PARP), and removal of PAR by poly(ADP-ribose) glycohydrolase (PARG). Three PARPs and two PARGs have been found in Arabidopsis, but their respective roles are not fully understood. In this study, the functions of each PARP and PARG in DNA repair were analyzed based on their mutant phenotypes under genotoxic stresses. Double or triple mutant analysis revealed that PARP1 and PARP2, but not PARP3, play a similar but not critical role in DNA repair in Arabidopsis seedlings. PARG1 and PARG2 play an essential and a minor role, respectively under the same conditions. Mutation of PARG1 results in increased DNA damage level and enhanced cell death in plants after bleomycin treatment. PARG1 expression is induced primarily in root and shoot meristems by bleomycin and induction of PARG1 is dependent on ATM and ATR kinases. PARG1 also antagonistically modulates the DNA repair process by preventing the over-induction of DNA repair genes. Our study determined the contribution of each PARP and PARG member in DNA repair and indicated that PARG1 plays a critical role in this process.

Original languageEnglish (US)
Article number15892
JournalScientific reports
Volume5
DOIs
StatePublished - Oct 30 2015

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DNA repair
ribose
cell viability
NAD ADP-ribosyltransferase
Arabidopsis
glycosidases
enzymes
mutants
root meristems
shoot meristems
post-translational modification
DNA damage
cell death
phosphotransferases (kinases)
proteins
mutation
phenotype
seedlings
genes

All Science Journal Classification (ASJC) codes

  • General

Cite this

Zhang, Hailei ; Gu, Zongying ; Wu, Qiao ; Yang, Lifeng ; Liu, Caifeng ; Ma, Hong ; Xia, Yiji ; Ge, Xiaochun. / Arabidopsis PARG1 is the key factor promoting cell survival among the enzymes regulating post-translational poly(ADP-ribosyl)ation. In: Scientific reports. 2015 ; Vol. 5.
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Arabidopsis PARG1 is the key factor promoting cell survival among the enzymes regulating post-translational poly(ADP-ribosyl)ation. / Zhang, Hailei; Gu, Zongying; Wu, Qiao; Yang, Lifeng; Liu, Caifeng; Ma, Hong; Xia, Yiji; Ge, Xiaochun.

In: Scientific reports, Vol. 5, 15892, 30.10.2015.

Research output: Contribution to journalArticle

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AU - Liu, Caifeng

AU - Ma, Hong

AU - Xia, Yiji

AU - Ge, Xiaochun

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