@article{cc07910924764531b9b5a41296905b85,
title = "Arabidopsis pavement cell morphogenesis requires FERONIA binding to pectin for activation of ROP GTPase signaling",
abstract = "Sensing and signaling of cell wall status and dynamics regulate many processes in plants, such as cell growth and morphogenesis, but the underpinning mechanisms remain largely unknown. Here, we demonstrate that the CrRLK1L receptor kinase FERONIA (FER) binds the cell wall pectin, directly leading to the activation of the ROP6 guanosine triphosphatase (GTPase) signaling pathway that regulates the formation of the puzzle piece shape of pavement cells in Arabidopsis. The extracellular malectin domain of FER binds demethylesterified pectin in vivo and in vitro. Both loss-of-FER mutations and defects in pectin demethylesterification caused similar changes in pavement cell shape and ROP6 GTPase signaling. FER is required for the activation of ROP6 by demethylesterified pectin and physically and genetically interacts with the ROP6 activator, RopGEF14. Thus, our findings elucidate a signaling pathway that directly connects the cell wall pectin to cellular morphogenesis via the cell surface receptor FER.",
author = "Wenwei Lin and Wenxin Tang and Xue Pan and Aobo Huang and Xiuqin Gao and Anderson, {Charles T.} and Zhenbiao Yang",
note = "Funding Information: We are grateful to Ueli Grossniklaus (University of Z{\"u}rich, Switzerland) for fer-2 seeds, to Vincenzo Lionetti (Sapienza University of Rome, Italy) for pme3 and 35Spro:PMEI1 transgenic seeds, and to Jia Li (University of Lanzhou, China) for pBAK1-BAK1-GFP seeds. We thank Eugene Nothnagel (University of California, Riverside, USA) and members of the Yang laboratory for their technical assistance and helpful discussions. C.T.A. is supported by the Center for Lignocellulose Structure and Formation , an Energy Frontier Research Center funded by the US Department of Energy, Office of Science, Basic Energy Sciences (award no. DE–SC0001090 ). This work is in part supported by a NIGMS grant ( GM100130 ) to Z.Y. and a grant from National Natural Science Foundation of China to W.T. (no. 31501138 ). Funding Information: We are grateful to Ueli Grossniklaus (University of Z{\"u}rich, Switzerland) for fer-2 seeds, to Vincenzo Lionetti (Sapienza University of Rome, Italy) for pme3 and 35Spro:PMEI1 transgenic seeds, and to Jia Li (University of Lanzhou, China) for pBAK1-BAK1-GFP seeds. We thank Eugene Nothnagel (University of California, Riverside, USA) and members of the Yang laboratory for their technical assistance and helpful discussions. C.T.A. is supported by the Center for Lignocellulose Structure and Formation, an Energy Frontier Research Center funded by the US Department of Energy, Office of Science, Basic Energy Sciences (award no. DE–SC0001090). This work is in part supported by a NIGMS grant (GM100130) to Z.Y. and a grant from National Natural Science Foundation of China to W.T. (no. 31501138). W.L. W.T. and Z.Y. designed the study. W.L. and W.T. performed the most experiments. X.P. performed the protoplast cell wall staining; X.P. A.H. and X.G. generated the constructs. W.L. C.T.A. and Z.Y. wrote the manuscript. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2021",
year = "2022",
month = feb,
day = "7",
doi = "10.1016/j.cub.2021.11.030",
language = "English (US)",
volume = "32",
pages = "497--507.e4",
journal = "Current Biology",
issn = "0960-9822",
publisher = "Cell Press",
number = "3",
}