Arabidopsis PTD Is Required for Type I Crossover Formation and Affects Recombination Frequency in Two Different Chromosomal Regions

Pingli Lu, Asela J. Wijeratne, Zhengjia Wang, Gregory P. Copenhaver, Hong Ma

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In eukaryotes, crossovers together with sister chromatid cohesion maintain physical association between homologous chromosomes, ensuring accurate chromosome segregation during meiosis I and resulting in exchange of genetic information between homologues. The Arabidopsis PTD (Parting Dancers) gene affects the level of meiotic crossover formation, but its functional relationships with other core meiotic genes, such as AtSPO11-1, AtRAD51, and AtMSH4, are unclear; whether PTD has other functions in meiosis is also unknown. To further analyze PTD function and to test for epistatic relationships, we compared the meiotic chromosome behaviors of Atspo11-1 ptd and Atrad51 ptd double mutants with the relevant single mutants. The results suggest that PTD functions downstream of AtSPO11-1 and AtRAD51 in the meiotic recombination pathway. Furthermore, we found that meiotic defects in rck ptd and Atmsh4 ptd double mutants showed similar meiotic phenotypes to those of the relevant single mutants, providing genetic evidences for roles of PTD and RCK in the type I crossovers pathway. Moreover, we employed a pollen tetrad-based fluorescence method and found that the meiotic crossover frequencies in two genetic intervals were significantly reduced from 6.63% and 22.26% in wild-type to 1.14% and 6.36%, respectively, in the ptd-2 mutant. These results revealed new aspects of PTD function in meiotic crossover formation.

Original languageEnglish (US)
Pages (from-to)165-175
Number of pages11
JournalJournal of Genetics and Genomics
Volume41
Issue number3
DOIs
StatePublished - Mar 20 2014

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Meiosis
Arabidopsis
Genetic Recombination
Chromosomes
Chromosome Segregation
Chromatids
Pollen
Eukaryota
Genes
Fluorescence
Phenotype

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Lu, Pingli ; Wijeratne, Asela J. ; Wang, Zhengjia ; Copenhaver, Gregory P. ; Ma, Hong. / Arabidopsis PTD Is Required for Type I Crossover Formation and Affects Recombination Frequency in Two Different Chromosomal Regions. In: Journal of Genetics and Genomics. 2014 ; Vol. 41, No. 3. pp. 165-175.
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abstract = "In eukaryotes, crossovers together with sister chromatid cohesion maintain physical association between homologous chromosomes, ensuring accurate chromosome segregation during meiosis I and resulting in exchange of genetic information between homologues. The Arabidopsis PTD (Parting Dancers) gene affects the level of meiotic crossover formation, but its functional relationships with other core meiotic genes, such as AtSPO11-1, AtRAD51, and AtMSH4, are unclear; whether PTD has other functions in meiosis is also unknown. To further analyze PTD function and to test for epistatic relationships, we compared the meiotic chromosome behaviors of Atspo11-1 ptd and Atrad51 ptd double mutants with the relevant single mutants. The results suggest that PTD functions downstream of AtSPO11-1 and AtRAD51 in the meiotic recombination pathway. Furthermore, we found that meiotic defects in rck ptd and Atmsh4 ptd double mutants showed similar meiotic phenotypes to those of the relevant single mutants, providing genetic evidences for roles of PTD and RCK in the type I crossovers pathway. Moreover, we employed a pollen tetrad-based fluorescence method and found that the meiotic crossover frequencies in two genetic intervals were significantly reduced from 6.63{\%} and 22.26{\%} in wild-type to 1.14{\%} and 6.36{\%}, respectively, in the ptd-2 mutant. These results revealed new aspects of PTD function in meiotic crossover formation.",
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Arabidopsis PTD Is Required for Type I Crossover Formation and Affects Recombination Frequency in Two Different Chromosomal Regions. / Lu, Pingli; Wijeratne, Asela J.; Wang, Zhengjia; Copenhaver, Gregory P.; Ma, Hong.

In: Journal of Genetics and Genomics, Vol. 41, No. 3, 20.03.2014, p. 165-175.

Research output: Contribution to journalArticle

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T1 - Arabidopsis PTD Is Required for Type I Crossover Formation and Affects Recombination Frequency in Two Different Chromosomal Regions

AU - Lu, Pingli

AU - Wijeratne, Asela J.

AU - Wang, Zhengjia

AU - Copenhaver, Gregory P.

AU - Ma, Hong

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