Arabidopsis sphingosine kinase and the effects of phytosphingosine-1- phosphate on stomatal aperture

Sylvie Coursol, Hervé Le Le Stunff, Daniel V. Lynch, Simon Gilroy, Sarah M. Assmann, Sarah Spiegel

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Sphingolipids are a major component of membrane lipids and their metabolite sphingosine-1-phosphate (S1P) is a potent lipid mediator in animal cells. Recently, we have shown that the enzyme responsible for S1P production, sphingosine kinase (SphK), is stimulated by the phytohormone abscisic acid in guard cells of Arabidopsis (Arabidopsis thaliana) and that S1P is effective in regulating guard cell turgor. We have now characterized SphK from Arabidopsis leaves. SphK activity was mainly associated with the membrane fraction and phosphorylated predominantly the Δ4-unsaturated long-chain sphingoid bases sphingosine (Sph) and 4,8-sphingadienine, and to a lesser extent, the saturated long-chain sphingoid bases dihydrosphingosine and phytosphingosine (Phyto-Sph). 4-Hydroxy-8-sphingenine, which is a major sphingoid base in complex glycosphingolipids from Arabidopsis leaves, was a relatively poor substrate compared with the corresponding saturated Phyto-Sph. In contrast, mammalian SphK1 efficiently phosphorylated Sph, dihydrosphingosine, and 4,8-sphingadienine, but not the 4-hydroxylated long-chain bases Phyto-Sph and 4-hydroxy-8-sphingenine. Surface dilution kinetic analysis of Arabidopsis SphK with Sph presented in mixed Triton X-100 micelles indicated that SphK associates with the micellar surface and then with the substrate presented on the surface. In addition, measurements of SphK activity under different assay conditions combined with phylogenetic analysis suggest that multiple isoforms of SphK may be expressed in Arabidopsis. Importantly, we found that phytosphingosine-1- phosphate, similar to S1P, regulates stomatal apertures and that its action is impaired in guard cells of Arabidopsis plants harboring T-DNA null mutations in the sole prototypical G-protein α-subunit gene, GPA1.

Original languageEnglish (US)
Pages (from-to)724-737
Number of pages14
JournalPlant physiology
Volume137
Issue number2
DOIs
StatePublished - Jan 1 2005

Fingerprint

sphingosine
phytosphingosine
Arabidopsis
phosphotransferases (kinases)
phosphates
Sphingosine
guard cells
Glycosphingolipids
Abscisic Acid
Sphingolipids
Plant Growth Regulators
Octoxynol
Protein Subunits
Plant Cells
Micelles
Membrane Lipids
phytosphingosine-1-phosphate
sphingosine kinase
GTP-Binding Proteins
Protein Isoforms

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

Coursol, Sylvie ; Le Stunff, Hervé Le ; Lynch, Daniel V. ; Gilroy, Simon ; Assmann, Sarah M. ; Spiegel, Sarah. / Arabidopsis sphingosine kinase and the effects of phytosphingosine-1- phosphate on stomatal aperture. In: Plant physiology. 2005 ; Vol. 137, No. 2. pp. 724-737.
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Arabidopsis sphingosine kinase and the effects of phytosphingosine-1- phosphate on stomatal aperture. / Coursol, Sylvie; Le Stunff, Hervé Le; Lynch, Daniel V.; Gilroy, Simon; Assmann, Sarah M.; Spiegel, Sarah.

In: Plant physiology, Vol. 137, No. 2, 01.01.2005, p. 724-737.

Research output: Contribution to journalArticle

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AU - Le Stunff, Hervé Le

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