Arabidopsis thaliana protein, ATK1, is a minus-end directed kinesin that exhibits non-processive movement

Adam I. Marcus, J. Christian Ambrose, Lisa Blickley, William O. Hancock, Richard J. Cyr

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The microtubule cytoskeleton forms the scaffolding of the meiotic spindle. Kinesins, which bind to microtubules and generate force via ATP hydrolysis, are also thought to play a critical role in spindle assembly, maintenance, and function. The A. thaliana protein, ATK1 (formerly known as KATA), is a member of the kinesin family based on sequence similarity and is implicated in spindle assembly and/or maintenance. Thus, we want to determine if ATK1 behaves as a kinesin in vitro, and if so, determine the directionality of the motor activity and processivity character (the relationship between molecular "steps" and microtubule association). The results show that ATK1 supports microtubule movement in an ATP-dependent manner and has a minus-end directed polarity. Furthermore, ATK1 exhibits non-processive movement along the microtubule and likely requires at least four ATK1 motors bound to the microtubule to support movement. Based on these results and previous data, we conclude that ATK1 is a non-processive, minus-end directed kinesin that likely plays a role in generating forces in the spindle during meiosis.

Original languageEnglish (US)
Pages (from-to)144-150
Number of pages7
JournalCell Motility and the Cytoskeleton
Volume52
Issue number3
DOIs
StatePublished - Jul 9 2002

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Arabidopsis Proteins
Kinesin
Microtubules
Adenosine Triphosphate
Maintenance
Spindle Apparatus
Meiosis
Cytoskeleton
Motor Activity
Hydrolysis

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Cell Biology

Cite this

Marcus, Adam I. ; Ambrose, J. Christian ; Blickley, Lisa ; Hancock, William O. ; Cyr, Richard J. / Arabidopsis thaliana protein, ATK1, is a minus-end directed kinesin that exhibits non-processive movement. In: Cell Motility and the Cytoskeleton. 2002 ; Vol. 52, No. 3. pp. 144-150.
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Arabidopsis thaliana protein, ATK1, is a minus-end directed kinesin that exhibits non-processive movement. / Marcus, Adam I.; Ambrose, J. Christian; Blickley, Lisa; Hancock, William O.; Cyr, Richard J.

In: Cell Motility and the Cytoskeleton, Vol. 52, No. 3, 09.07.2002, p. 144-150.

Research output: Contribution to journalArticle

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