Architecture-dependent collective calcium signaling in microengineered and self-organized endothelial cell networks

J. Sun, P. K. Wong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper investigates the architecture dependence of endothelial cell networks on collective calcium signaling. Upon exogenous histamine stimulation, differential calcium responses are observed in human umbilical vein endothelial cells (IIUVEC) cultured monolayers, plasma lithography patterned hexagon cell networks, and capillary-like structures (CLS) self-organized on matrigel. By systematically modulating the cell-cell interaction with geometrical patterning and the gap-junction intercellular communication with pharmacological reagents, our results reveal the emergent network property of endothelial cells in response to extracellular chemical signals.

Original languageEnglish (US)
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages428-430
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - Jan 1 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume1

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period10/27/1310/31/13

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All Science Journal Classification (ASJC) codes

  • Bioengineering

Cite this

Sun, J., & Wong, P. K. (2013). Architecture-dependent collective calcium signaling in microengineered and self-organized endothelial cell networks. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 428-430). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 1). Chemical and Biological Microsystems Society.