Architecture of a dsDNA viral capsid in complex with its maturation protease

David Veesler, Reza Khayat, Srinath Krishnamurthy, Joost Snijder, Rick K. Huang, Albert J.R. Heck, Ganesh S. Anand, John E. Johnson

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Most double-stranded DNA (dsDNA) viruses, including bacteriophages and herpesviruses, rely on a staged assembly process of capsid formation. A viral protease is required for many of them to disconnect scaffolding domains/proteins from the capsid shell, therefore priming the maturation process. We used the bacteriophage HK97 as a model system to decipher the molecular mechanisms underlying the recruitment of the maturation protease by the assembling procapsid and the influence exerted onto the latter. Comparisons of the procapsid with and without protease using single-particle cryoelectron microscopy reconstructions, hydrogen/deuterium exchange coupled to mass spectrometry, and native mass spectrometry demonstrated that the protease interacts with the scaffolding domains within the procapsid interior and stabilizes them as well as the whole particle. The results suggest that the thermodynamic consequences of protease packaging are to shift the equilibrium between isolated coat subunit capsomers and procapsid in favor of the latter by stabilizing the assembled particle before making the process irreversible through proteolysis of the scaffolding domains.

Original languageEnglish (US)
Pages (from-to)230-237
Number of pages8
JournalStructure
Volume22
Issue number2
DOIs
StatePublished - Feb 4 2014

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

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