Architecture of the vimentin cytoskeleton is modified by perturbation of the GTPase ARF1

Melanie L. Styers, Andrew P. Kowalczyk, Victor Faundez

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Intermediate filaments are required for proper membrane protein trafficking. However, it remains unclear whether perturbations in vesicular membrane transport result in changes in the architecture of the vimentin cytoskeleton. We find that treatment of cells with Brefeldin A, an inhibitor of specific stages of membrane transport, causes changes in the organization of vimentin filaments. These changes arise from movement of pre-existing filaments. Brefeldin A treatment also leads to alterations in the microtubule cytoskeleton. However, this effect is not observed in cells lacking intermediate filaments, indicating that microtubule bundling is downstream of perturbations in the vimentin cytoskeleton. Brefeldin A-induced changes in vimentin architecture are probably mediated through its effects on ADP-ribosylation factor 1 (ARF1). Expression of a dominant-negative mutant of ARF1 induces BFA-like modifications in vimentin morphology. The BFA-dependent changes in vimentin architecture occurred concurrently with the release of the ARF1-regulated adaptor complexes AP-3 and AP-1 from membranes and adaptor redistribution to vimentin networks. These observations indicate that perturbation of the vesicular membrane transport machinery lead to reciprocal changes in the architecture of vimentin networks.

Original languageEnglish (US)
Pages (from-to)3643-3654
Number of pages12
JournalJournal of Cell Science
Volume119
Issue number17
DOIs
StatePublished - Sep 1 2006

All Science Journal Classification (ASJC) codes

  • Cell Biology

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