Arterial and venous smooth muscle cell proliferation in response to co- culture with arterial and venous endothelial cells

Peter Waybill, L. Joseph Hopkins

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

PURPOSE: To determine whether definable differences exist between arterial and venous smooth muscle cells (SMCs), as measured by proliferative response to co-culture with arterial or venous endothelial cells (ECs). MATERIALS AND METHODS: Human aortic ECs (A-ECs) and saphenous vein ECs (V- ECs) were cultured opposite either aortic SMCs (A-SMCs) or saphenous vein SMCs (V-SMCs). At selected time intervals, SMCs were counted by fluorescence microscopy. RESULTS: In the presence of an intact EC monolayer, A-ECs induced a 9%-31% increase in A-SMC (P ≤ .001) and a 15%-37% increase in V-SMC (P ≤ .001) proliferation. Saphenous vein ECs induced a 50%71% increase in A-SMC (P ≤ .001) and a 40%-62% increase in V-SMC (P ≤ .001) proliferation. The small proliferative difference between A-SMCs and V-SMCs was significant for co- culture with A-ECs (P ≤ .001) and V-ECs (P ≤ .001). Of note, compared to A- ECs, V-ECs induced a significantly greater A-SMC (P ≤ .001) and V-SMC (P ≤ .001) proliferative response. CONCLUSION: A small, but definable, difference exists between A-SMCs and V-SMCs, as measured by proliferative response in coculture with A-ECs and V-ECs.

Original languageEnglish (US)
Pages (from-to)1051-1057
Number of pages7
JournalJournal of Vascular and Interventional Radiology
Volume10
Issue number8
DOIs
StatePublished - 1999

All Science Journal Classification (ASJC) codes

  • Radiology Nuclear Medicine and imaging
  • Cardiology and Cardiovascular Medicine

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