Arterial macrophages and regenerating endothelial cells express P-selectin in atherosclerosis-prone apolipoprotein E-deficient mice

Guohong Li, John M. Sanders, Elizabeth T. Phan, Klaus Ley, Ian J. Sarembock

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

P-selectin expression has been reported in platelets, endothelial cells, and vascular smooth muscle cells in response to vascular injury. Here, we report P-selectin expression on macrophages in the arterial wall after carotid denudation injury and spontaneous atherosclerosis in atherosclerosis-prone apoE-deficient (apoE-/-) mice. Double-immunofluorescence staining revealed robust P-selectin expression in macrophage-rich regions of both denudation-induced carotid neointimal lesions and innominate atherosclerotic plaques. Co-localization of P-selectin with macrophages was verified at the single cell level using double immunostaining plus 4,6-diamidino-2-phenylindole (for nuclei) counter-staining. No platelet staining was seen in association with the macrophage staining, excluding platelet contamination. Furthermore, P-selectin mRNA expression was readily detectable in macrophage-rich plaques of atherosclerotic innominate arteries and blood monocyte-derived macrophages from apoE-/- mice. Strong P-selectin expression was also seen in the areas of regenerated endothelium after arterial injury. In addition, co-localization of P-selectin with vascular smooth muscle cells was readily observed in denudation-injured carotid arteries at 7 and 14 days. We conclude that macrophages in carotid injury-induced neointimal lesions and spontaneous atherosclerotic plaques of the innominate artery acquire the ability to express P-selectin, as does regenerating endothelium. These findings provide a potential new paradigm in macrophage-mediated vascular inflammation, atherosclerosis, and neointimal hyperplasia after arterial injury.

Original languageEnglish (US)
Pages (from-to)1511-1518
Number of pages8
JournalAmerican Journal of Pathology
Volume167
Issue number6
DOIs
StatePublished - Dec 2005

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P-Selectin
Apolipoproteins E
Atherosclerosis
Endothelial Cells
Macrophages
Atherosclerotic Plaques
Staining and Labeling
Brachiocephalic Trunk
Blood Platelets
Wounds and Injuries
Vascular Smooth Muscle
Smooth Muscle Myocytes
Endothelium
Vascular System Injuries
Carotid Arteries
Hyperplasia
Fluorescent Antibody Technique
Blood Vessels
Inflammation
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Li, Guohong ; Sanders, John M. ; Phan, Elizabeth T. ; Ley, Klaus ; Sarembock, Ian J. / Arterial macrophages and regenerating endothelial cells express P-selectin in atherosclerosis-prone apolipoprotein E-deficient mice. In: American Journal of Pathology. 2005 ; Vol. 167, No. 6. pp. 1511-1518.
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Arterial macrophages and regenerating endothelial cells express P-selectin in atherosclerosis-prone apolipoprotein E-deficient mice. / Li, Guohong; Sanders, John M.; Phan, Elizabeth T.; Ley, Klaus; Sarembock, Ian J.

In: American Journal of Pathology, Vol. 167, No. 6, 12.2005, p. 1511-1518.

Research output: Contribution to journalArticle

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