Asbestos increases mammalian AP-endonuclease gene expression, protein levels, and enzyme activity in mesothelial cells

Hua Fung, Yoke W. Kow, Bennett Van Houten, Douglas J. Taatjes, Zafer Hatahet, Yvonne M.W. Janssen, Pam Vacek, Stephen P. Faux, Brooke T. Mossman

Research output: Contribution to journalArticle

79 Scopus citations

Abstract

Only two DNA repair enzymes, DNA polymecase β and O6-methylguanine- DNA methyltransferase, have been shown to be inducible in mammalian cells by genotoxic agents. We show here that crocidolite asbestos induces the DNA repair enzyme, apurinic/apyrimidinic (AP)-endonuclease, in isolated mesothelial cells, the progenitor cells of malignant mesothetioma. Asbestos at nontoxic concentrations of 1.25 and 2.5 μg/cm2 significantly increased AP-endonuclease mRNA and protein levels as well as enzyme activity (P < 0.05) in a dose-dependent manner in rat pleural mesothelial cells. These increases were persistent from 24 to 72 h after initial exposure to fibers. Changes were not observed with glass beads, a noncarcinogenic particle. Confocal scanning laser microscopy showed that AP-endonuclease was primarily localized in the nucleus but also in mitochondria. Our data are the first to demonstrate the inducibility of AP-endonuclease by a human class I carcinogen associated with oxidant stress in normal cells of the lung.

Original languageEnglish (US)
Pages (from-to)189-194
Number of pages6
JournalCancer Research
Volume58
Issue number2
StatePublished - Jan 15 1998

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Asbestos increases mammalian AP-endonuclease gene expression, protein levels, and enzyme activity in mesothelial cells'. Together they form a unique fingerprint.

  • Cite this

    Fung, H., Kow, Y. W., Van Houten, B., Taatjes, D. J., Hatahet, Z., Janssen, Y. M. W., Vacek, P., Faux, S. P., & Mossman, B. T. (1998). Asbestos increases mammalian AP-endonuclease gene expression, protein levels, and enzyme activity in mesothelial cells. Cancer Research, 58(2), 189-194.