Abstract
The effect of ascorbic acid (AA) on rat glioma C6 cells was studied. At physiological AA concentrations of 0.1 and 1 mM, no morphological and no proliferative alterations in the C6 cultures were detectable. Although the total RNA content per cell was not affected by the AA-treatment, AA upregulated the expression of myelin-specific genes, i.e. proteolipid protein (PLP) and myelin-associated glycoprotein (MAG) genes as assessed by northern blot analysis. The steady-state level of the specific mRNAs increased transiently in the AA-treated cells. Three days after AA administration the message level reached a maximum of 10-and 2-fold over control for the PLP and MAG genes, respectively. The upregulation of the genes was directly related to AA concentration. The present data indicate a possible involvement of AA in the regulation of myelin gene activity in the CNS.
Original language | English (US) |
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Pages (from-to) | 157-164 |
Number of pages | 8 |
Journal | Metabolic Brain Disease |
Volume | 7 |
Issue number | 3 |
DOIs | |
State | Published - Sep 1992 |
All Science Journal Classification (ASJC) codes
- Biochemistry
- Clinical Neurology
- Cellular and Molecular Neuroscience