Ascorbic acid upregulates myelin gene expression in C6 glioma cells

I. Laszkiewicz; R. C. Wiggins; G. Konat

doi:10.1007/BF01000161

Ascorbic acid upregulates myelin gene expression in C6 glioma cells

I. Laszkiewicz, R. C. Wiggins, G. Konat

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The effect of ascorbic acid (AA) on rat glioma C6 cells was studied. At physiological AA concentrations of 0.1 and 1 mM, no morphological and no proliferative alterations in the C6 cultures were detectable. Although the total RNA content per cell was not affected by the AA-treatment, AA upregulated the expression of myelin-specific genes, i.e. proteolipid protein (PLP) and myelin-associated glycoprotein (MAG) genes as assessed by northern blot analysis. The steady-state level of the specific mRNAs increased transiently in the AA-treated cells. Three days after AA administration the message level reached a maximum of 10-and 2-fold over control for the PLP and MAG genes, respectively. The upregulation of the genes was directly related to AA concentration. The present data indicate a possible involvement of AA in the regulation of myelin gene activity in the CNS.

Original language	English (US)
Pages (from-to)	157-164
Number of pages	8
Journal	Metabolic Brain Disease
Volume	7
Issue number	3
DOIs	https://doi.org/10.1007/BF01000161
State	Published - Sep 1992

All Science Journal Classification (ASJC) codes

Biochemistry
Clinical Neurology
Cellular and Molecular Neuroscience

Access to Document

10.1007/BF01000161

Cite this

@article{f41e5b1dce1a4499a315c051e38b583f,

title = "Ascorbic acid upregulates myelin gene expression in C6 glioma cells",

abstract = "The effect of ascorbic acid (AA) on rat glioma C6 cells was studied. At physiological AA concentrations of 0.1 and 1 mM, no morphological and no proliferative alterations in the C6 cultures were detectable. Although the total RNA content per cell was not affected by the AA-treatment, AA upregulated the expression of myelin-specific genes, i.e. proteolipid protein (PLP) and myelin-associated glycoprotein (MAG) genes as assessed by northern blot analysis. The steady-state level of the specific mRNAs increased transiently in the AA-treated cells. Three days after AA administration the message level reached a maximum of 10-and 2-fold over control for the PLP and MAG genes, respectively. The upregulation of the genes was directly related to AA concentration. The present data indicate a possible involvement of AA in the regulation of myelin gene activity in the CNS.",

author = "I. Laszkiewicz and Wiggins, {R. C.} and G. Konat",

year = "1992",

month = sep,

doi = "10.1007/BF01000161",

language = "English (US)",

volume = "7",

pages = "157--164",

journal = "Metabolic Brain Disease",

issn = "0885-7490",

publisher = "Springer New York",

number = "3",

}

TY - JOUR

T1 - Ascorbic acid upregulates myelin gene expression in C6 glioma cells

AU - Laszkiewicz, I.

AU - Wiggins, R. C.

AU - Konat, G.

PY - 1992/9

Y1 - 1992/9

N2 - The effect of ascorbic acid (AA) on rat glioma C6 cells was studied. At physiological AA concentrations of 0.1 and 1 mM, no morphological and no proliferative alterations in the C6 cultures were detectable. Although the total RNA content per cell was not affected by the AA-treatment, AA upregulated the expression of myelin-specific genes, i.e. proteolipid protein (PLP) and myelin-associated glycoprotein (MAG) genes as assessed by northern blot analysis. The steady-state level of the specific mRNAs increased transiently in the AA-treated cells. Three days after AA administration the message level reached a maximum of 10-and 2-fold over control for the PLP and MAG genes, respectively. The upregulation of the genes was directly related to AA concentration. The present data indicate a possible involvement of AA in the regulation of myelin gene activity in the CNS.

AB - The effect of ascorbic acid (AA) on rat glioma C6 cells was studied. At physiological AA concentrations of 0.1 and 1 mM, no morphological and no proliferative alterations in the C6 cultures were detectable. Although the total RNA content per cell was not affected by the AA-treatment, AA upregulated the expression of myelin-specific genes, i.e. proteolipid protein (PLP) and myelin-associated glycoprotein (MAG) genes as assessed by northern blot analysis. The steady-state level of the specific mRNAs increased transiently in the AA-treated cells. Three days after AA administration the message level reached a maximum of 10-and 2-fold over control for the PLP and MAG genes, respectively. The upregulation of the genes was directly related to AA concentration. The present data indicate a possible involvement of AA in the regulation of myelin gene activity in the CNS.

UR - http://www.scopus.com/inward/record.url?scp=0026699323&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026699323&partnerID=8YFLogxK

U2 - 10.1007/BF01000161

DO - 10.1007/BF01000161

M3 - Article

C2 - 1279371

AN - SCOPUS:0026699323

VL - 7

SP - 157

EP - 164

JO - Metabolic Brain Disease

JF - Metabolic Brain Disease

SN - 0885-7490

IS - 3

ER -

Ascorbic acid upregulates myelin gene expression in C6 glioma cells

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this