Aspartic proteinase content of the arabidopsis genome

Carlos Faro, Susannah Gal

Research output: Contribution to journalReview article

41 Citations (Scopus)

Abstract

The sequence of the Arabidopsis genome has given us information about one plant's complement of aspartic proteinases. Using an in silico analysis based on the homology to known aspartic proteinase genes, we have uncovered 51 sequences that potentially encode these enzymes. This is substantial more than the number predicted for other eukaryotic systems. We have grouped the deduced amino acid sequences into 3 classes - typical plant aspartic proteinase, nucellin-like and atypical aspartic proteinase sequences-, depending on their putative domain organizations and their active site sequence motifs. Searching databases has revealed cDNAs or ESTs for nearly 90% of these genes. Sequence analysis using software that detects targeting signals indicates most of the predicted proteins have the expected localization in the secretory system although several of these are membrane bound. The analysis also predicts 8 chloroplast localized proteins and 2 mitochondria-localized aspartic proteinase-like proteins. The wide variety of structures and subcellular locations implies multiple functions for aspartic proteinases in plants.

Original languageEnglish (US)
Pages (from-to)493-500
Number of pages8
JournalCurrent Protein and Peptide Science
Volume6
Issue number6
DOIs
StatePublished - Dec 2005

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Aspartic Acid Proteases
Arabidopsis
Genes
Genome
Chloroplast Proteins
Proteins
Mitochondria
Expressed Sequence Tags
Computer Simulation
Sequence Analysis
Amino Acid Sequence
Catalytic Domain
Software
Complementary DNA
Databases
Membranes
Amino Acids
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "The sequence of the Arabidopsis genome has given us information about one plant's complement of aspartic proteinases. Using an in silico analysis based on the homology to known aspartic proteinase genes, we have uncovered 51 sequences that potentially encode these enzymes. This is substantial more than the number predicted for other eukaryotic systems. We have grouped the deduced amino acid sequences into 3 classes - typical plant aspartic proteinase, nucellin-like and atypical aspartic proteinase sequences-, depending on their putative domain organizations and their active site sequence motifs. Searching databases has revealed cDNAs or ESTs for nearly 90{\%} of these genes. Sequence analysis using software that detects targeting signals indicates most of the predicted proteins have the expected localization in the secretory system although several of these are membrane bound. The analysis also predicts 8 chloroplast localized proteins and 2 mitochondria-localized aspartic proteinase-like proteins. The wide variety of structures and subcellular locations implies multiple functions for aspartic proteinases in plants.",
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Aspartic proteinase content of the arabidopsis genome. / Faro, Carlos; Gal, Susannah.

In: Current Protein and Peptide Science, Vol. 6, No. 6, 12.2005, p. 493-500.

Research output: Contribution to journalReview article

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