Aspartic proteinase genes in the brassicaceae Arabidopsis thaliana and Brassica napus

Kathleen D'Hondt, Sylvia Stack, Steve Gutteridge, Joel Vandekerckhove, Enno Krebbers, Susannah Gal

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Active aspartic proteinase is isolated from Brassica napus seeds and the peptide sequence is used to generate primers for PCR. We present here cDNA and genomic clones for aspartic proteinases from the closely related Brassicaceae Arabidopsis thaliana and Brassica napus. The Arabidopsis cDNA represents a single gene, while Brassica has at least 4 genes. Like other plant aspartic proteases, the two Brassicaceae enzymes contain an extra protein domain of about 100 amino acids relative to the mammalian forms. The intron/exon arrangement in the Brassica genomic clone is significantly different from that in mammalian genes. As the proteinase is isolated from seeds, the same tissue where 2S albumins are processed, this implies expression of one of the aspartic proteinase genes there.

Original languageEnglish (US)
Pages (from-to)187-192
Number of pages6
JournalPlant molecular biology
Volume33
Issue number1
DOIs
StatePublished - Jan 1 1997

Fingerprint

Brassicaceae
Aspartic Acid Proteases
aspartic proteinases
Brassica napus
Arabidopsis
Arabidopsis thaliana
Genes
Brassica
Seed
Seeds
Peptide Hydrolases
genes
Complementary DNA
Clone Cells
clones
genomics
seeds
Introns
exons
albumins

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

D'Hondt, Kathleen ; Stack, Sylvia ; Gutteridge, Steve ; Vandekerckhove, Joel ; Krebbers, Enno ; Gal, Susannah. / Aspartic proteinase genes in the brassicaceae Arabidopsis thaliana and Brassica napus. In: Plant molecular biology. 1997 ; Vol. 33, No. 1. pp. 187-192.
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D'Hondt, K, Stack, S, Gutteridge, S, Vandekerckhove, J, Krebbers, E & Gal, S 1997, 'Aspartic proteinase genes in the brassicaceae Arabidopsis thaliana and Brassica napus', Plant molecular biology, vol. 33, no. 1, pp. 187-192. https://doi.org/10.1023/A:1005794917200

Aspartic proteinase genes in the brassicaceae Arabidopsis thaliana and Brassica napus. / D'Hondt, Kathleen; Stack, Sylvia; Gutteridge, Steve; Vandekerckhove, Joel; Krebbers, Enno; Gal, Susannah.

In: Plant molecular biology, Vol. 33, No. 1, 01.01.1997, p. 187-192.

Research output: Contribution to journalArticle

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